| 赵晓平,刘晓光.2011.实验室薄口螨的大量培养及休眠体的诱导.动物学杂志,46(4):42-46. |
| 实验室薄口螨的大量培养及休眠体的诱导 |
| Mass Culture of Histiostoma laboratorium and Their Hypopi Induction |
| 投稿时间：2010-10-30 修订日期：2011-04-11 |
| DOI： |
| 中文关键词: 实验室薄口螨 培养 休眠体 诱导 |
| 英文关键词:Histiostoma laboratorium Culture Hypopus Induction |
| 基金项目:内蒙古自治区高等学校科学研究项目(No.NJ09151) |
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| 中文摘要: |
| 用玉米粉培养基、BY(牛肉膏＋酵母膏)软琼脂培养基、BY培养液3种培养基对实验室薄口螨(Histiostoma laboratorium)进行了培养。其最适培养基是玉米粉培养基;该螨在BY软琼脂培养基上也能生长,但生长速度比较缓慢,经过BY软琼脂培养基的培养,能够收集到大量干净的个体,为DNA提取和分子生物学研究提供了方便;在BY培养液中,实验室薄口螨不能进行继代生长,但能够产生大量的卵,可收集卵做更进一步的深入研究。休眠体是该螨生活史中的重要阶段,是借助携播者进行传播的特殊形式。对孳生于培养有果蝇的玻璃指管中的实验室薄口螨产生的休眠体及其在果蝇体表的吸附状况进行了观察,利用较高温度(30~35℃)培养基逐步干燥、较低温度(10~15℃)、BY液体培养3种方法,可以诱导该螨休眠体集中大量地形成,方便收集休眠体,是对其进行生理生化、基因表达、疾病传播机理等方面研究的先决条件。 |
| 英文摘要: |
| Histiostoma laboratorium Hughes individuals were cultured in corn powder culture medium, BY(beef extract＋yeast extract)soft agar culture medium, and BY (beef extract＋yeast extract) culture liquid. The most suitable culture medium for the mites was corn powder culture medium; they grew more slowly when culturing in BY soft agar culture medium than in corn powder culture medium but numerous clean individuals could be collected for DNA extraction and molecular biology research. Although H.laboratorium Hughes could not grow generation by generation in BY culture liquid, they could produce numerous ova which could be collected for research. Hypopi were specialized forms of the mites to disperse by means of other animals. Hypopi of H.laboratorium Hughes could propagate in thin glass pipe where fruit flies had been existed as well as on the body of fruit flies. Several methods including progressively drying of culture medium at high temperature (30-35℃),culture at lower temperature(10-15℃),and liquid culture could induce H.laboratorium to form hypopi mass that could be collected for its physiological, biochemical, gene expression, disease spreading mechanism and other researches. |
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