Histiostoma laboratorium Hughes individuals were cultured in corn powder culture medium, BY(beef extract＋yeast extract)soft agar culture medium, and BY (beef extract＋yeast extract) culture liquid. The most suitable culture medium for the mites was corn powder culture medium; they grew more slowly when culturing in BY soft agar culture medium than in corn powder culture medium but numerous clean individuals could be collected for DNA extraction and molecular biology research. Although H.laboratorium Hughes could not grow generation by generation in BY culture liquid, they could produce numerous ova which could be collected for research. Hypopi were specialized forms of the mites to disperse by means of other animals. Hypopi of H.laboratorium Hughes could propagate in thin glass pipe where fruit flies had been existed as well as on the body of fruit flies. Several methods including progressively drying of culture medium at high temperature (30-35℃),culture at lower temperature(10-15℃),and liquid culture could induce H.laboratorium to form hypopi mass that could be collected for its physiological, biochemical, gene expression, disease spreading mechanism and other researches.