| 王宜艳,宋晓娜,付静芸,孙虎山.2011.贝类血细胞硫代黄素T荧光染色方法.动物学杂志,46(6):40-45. |
| 贝类血细胞硫代黄素T荧光染色方法 |
| The Fluorescent Staining of Mollusks Haemocytes with Thioflavin T |
| 投稿时间：2011-05-18 修订日期：2011-09-02 |
| DOI： |
| 中文关键词: 贝类 血细胞 硫代黄素T 荧光染色 |
| 英文关键词:Mollusks Haemocytes Thioflavin T Fluorescence staining |
| 基金项目:山东省自然科学基金项目(No.ZR2010CM017) |
|
| 摘要点击次数: 2614 |
| 全文下载次数: 2709 |
| 中文摘要: |
| 硫代黄素T(thioflavin T,TFT)是一种用于组织学的苯并噻唑荧光染料,因其对淀粉样蛋白有高亲和性而主要被用于淀粉样病变的荧光显微检测。本研究分别以软体动物门双壳纲的栉孔扇贝(Chlamys farreri)和中国蛤蜊(Mactra chinensis)、腹足纲的拟紫口玉螺(Natica janthostomoides)及头足纲的短蛸(Octopus variabilis)4种软体动物为实验材料,对贝类血细胞的TFT染色方法进行了探讨。4种贝类血细胞经0.1%的TFT染色后,荧光显微镜下多呈黄绿色荧光,细胞轮廓清晰、细胞核及胞质中的颗粒与细胞质背景区分明显。4种贝类的血细胞均可根据细胞质内是否存在颗粒区分为无颗粒细胞和颗粒细胞两大类型,其中栉孔扇贝、中国蛤蜊及短蛸的无颗粒细胞可分为透明细胞和成血细胞两种亚型;颗粒细胞根据其颗粒的大小可分为小颗粒细胞和大颗粒细胞两种亚型。贝类血细胞TFT染色方法简单易操作,染色后荧光不易减弱,其标本可供长时间的观察。本研究首次尝试将TFT用于贝类血细胞的形态观察及类型区分的研究,表明TFT荧光染色是贝类血细胞观察与分类的一种好方法。 |
| 英文摘要: |
| Thioflavin (TFT), a benzothiazole fluorescent dye, is able to selectively stain amyloid structures and is mainly used to detect amyloidosis using fluorescence microscopy. In this study, the circulating hemocytes of four species of mollusks, Chlamys farreri (Bivalvia), Mactra chinensis (Bivalvia), Natica janthostomoides (Gastropoda) and Octopus variabilis (Cephalopoda), were observed in order to develop the fluorescence staining method of mollusks haemocytes with TFT. Under fluorescence microscope, haemocytes of four mollusks showed yellow-green fluorescence after treatment with 0.1% TFT. The outlines of the cells were clear; the nuclei and granules in the cytoplasm were clearly distinguishable. The haemocytes of all four mollusks could be divided into two types, granulocytes and agranulocytes, based on whether cytoplasmic granules could be observed. In scallop, clam and octopus, the agranulocytes could be subdivided into hyalinocytes and haemoblasts, while the granulocytes could be subdivided into small granulocytes and large granulocytes according to the size of granules in the cytoplasm. The fluorescence staining method of mollusks haemocytes with TFT is easy to operate, and the samples can be observed for a long time after staining because the fluorescence is not easy to bleach. This study is the first to use TFT in the morphological characterization and classification of mollusks haemocytes, and suggests that TFT fluorescence staining is a good method for the observation and classification of mollusks haemocytes. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
