| 叶平,宋金辉,侯连生.2011.细胞色素c在盘基网柄菌细胞凋亡中的作用.动物学杂志,46(6):73-79. |
| 细胞色素c在盘基网柄菌细胞凋亡中的作用 |
| Role of Cytochrome c in Apoptosis of Dictyostelium discoideum |
| 投稿时间：2011-05-03 修订日期：2011-09-20 |
| DOI： |
| 中文关键词: 盘基网柄菌 细胞色素c 线粒体 细胞凋亡 |
| 英文关键词:Dictyostelium discoideum Cytochrome c Mitochondrial Apoptosis |
| 基金项目:国家自然科学基金项目(No.30970316) |
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| 中文摘要: |
| 细胞色素c在细胞凋亡中发挥着重要的作用,其作用机理在高等真核生物及低等真核生物酵母中已经比较清楚,但在盘基网柄菌(Dictyostelium discoideum)中的作用却没有相关报道。所以我们用western blot和实时荧光定量PCR的方法分别测定了盘基网柄菌前柄细胞和前孢子细胞中细胞色素c的含量及表达量的变化,来探讨细胞色素c在盘基网柄菌前柄细胞凋亡中的作用。结果显示,1凋亡的前柄细胞发育16~20 h的过程中,线粒体内细胞色素c含量呈递减趋势,与之相反的是胞质中细胞色素c的含量却呈现出递增趋势。前柄细胞细胞色素c总含量高于前孢子细胞。2前柄细胞中细胞色素c基因的表达水平明显高于前孢子细胞,并且呈现出递增的趋势,而在前孢子细胞中细胞色素c基因的表达量比较稳定。两个研究结果相互印证,说明细胞色素c在一定程度上参与了盘基网柄菌前柄细胞的凋亡过程。 |
| 英文摘要: |
| Cytochrome c plays an important role in apoptosis of eukaryotes and yeast, but there are few reports about its role in Dictyostelium discoideum. Western blot and S Fluorescence Quantitative PCR were used to detect the quantity and expression changes of cytochrome c in prestalk cells and prespore cells, in order to reveal the role of cytochrome c in apoptosis of dityostelium prestalk cells. In process of 16-20 h development of apoptotic prestalk cells, the amount of mitochondrial cytochrome c showed a decreasing tendency while the amount of cytoplasmic cytochrome c showed an increasing tendency inversely. Total amount of cytochrome c in prestalk cells was higher than that in prespore cells. Cytochrome c gene expression in prestalk cells was higher than that in prespore cells, and showed an increasing tendency, but cytochrome c gene expression in prespore cell was relatively stable. These results indicate that cytochrome c, to some extent, participates in the apoptosis of Dictyostelium prestalk cells. |
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