| 雷飞宙,江建平,李成,谢锋.2012.角蟾亚科3物种皮肤的组织学观察.动物学杂志,47(3):20-27. |
| 角蟾亚科3物种皮肤的组织学观察 |
| Histological Observation of Skin from Three Species of Megophryinae |
| 投稿时间：2011-12-23 修订日期：2012-03-08 |
| DOI： |
| 中文关键词: 角蟾亚科 皮肤 钙化层 皮肤骨化 |
| 英文关键词:Megophryinae Skin Lamina calcarea Osteoderms |
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| 中文摘要: |
| 应用石蜡切片和H.E染色技术,对角蟾亚科3个属的代表物种:短肢异角蟾(Xenophrys brachykolos)、宽头短腿蟾(Brachytarsophrys carinense)和小口拟角蟾(Ophryophryne microstoma)的皮肤进行了组织学观察及参数测量比较。分别取头背、体背和体腹3个部位的皮肤进行观察。结果表明,3物种的皮肤基本结构相似,均由表皮和真皮组成,真皮包括疏松层和致密层,疏松层中有大量腺体分布,包括黏液腺和颗粒腺2种。皮肤厚度、各组织层相对厚度以及腺体密度之间存在种间差异和部位差异。在宽头短腿蟾背部皮肤中,发现了与尖吻山角蟾(Megophrys nasuta)皮肤中一种片层状、H.E染色呈蓝色的皮肤真皮骨化结构(osteoderms)很相似的结构;短肢异角蟾皮肤中有明显的钙化层结构,小口拟角蟾皮肤钙化程度较弱。皮肤的骨化和钙化可能具有防止水分流失,抵御干燥的功能。2种内骨骼在角蟾亚科中同时存在,为探讨两栖动物皮肤内骨骼的进化提供了重要的研究基础。 |
| 英文摘要: |
| We observed the skin structures of Xenophrys brachykolos, Brachytarsophrys carinense and Ophryophryne microstoma, representing three genera of Megophryinae respectively, using paraffin section and hematoxylin-eosin staining.Skins taken from dorsal head, dorsal and ventral trunk were observed.The skin structures of three species were similar, composed of epidermis and dermis.Dermis was made up of stratum spongiosum and stratum compactum.The stratum spongiosum abounded with two types of glands: mucus gland and granular gland.However, there were interspecific difference and inter-position difference in skin thickness and glands density.Osteoderms, which had laminate structure and were blue after staining with hematoxylin-eosin, were found in the skin of B.carinense, while the lamina calcarea was found in the skins of X.brachykolos and O.microstoma.However, the lamina calcarea was more developed in the skin of X.brachykolos than in O.microstoma.The functions of both structures of osteoderms and lamina calcarea could be water conservation and anti-desiccation.They exist in the Megophryinae, providing important clues for the research of integumentary skeleton evolution. |
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