唐琳,王文彬,曾伯平,席在星,王钦.2012.黄鳝体内寄生胃瘤线虫的染色体核型及G-带分析.动物学杂志,47(4):68-73. |
黄鳝体内寄生胃瘤线虫的染色体核型及G-带分析 |
Analysis of Karyotype and G-Banding Pattern of the Parasitic Nematode Eustrongylides ignotus in the Ricefield Eel Monopterus albus |
投稿时间:2012-02-28 修订日期:2012-04-20 |
DOI: |
中文关键词: 胃瘤线虫 黄鳝 染色体 核型 G-带 |
英文关键词:Eustrongylides ignotus Monopterus albus Chromosome Karyotype G-banding |
基金项目:湖南省自然科学基金项目(No.06JJ5036);湖南省教育厅科研项目(No.09C697);湖南省高校科技创新团队支持计划和湖南省"十二五"重点建设学科(动物学)资助; |
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中文摘要: |
采用常规空气干燥法制片,对寄生于黄鳝(Monopterus albus)体腔内的胃瘤线虫(Eustrongylides ignotus)染色体核型进行分析。结果表明:胃瘤线虫体细胞有12条染色体,为二倍体,核型公式为2n=12=10 m+2 sm。由5对常染色体和1对性染色体组成,性别决定模式为XX-XY,其中X、Y和1~4号染色体都为中着丝粒染色体,5号为亚中着丝粒染色体。每对染色体都有特定的G-带带型。 |
英文摘要: |
The karyotype of the larvae Eustrongylides ignotus, a parasitic nematode of Monopterus albus, collected from Changde City of Hunan Province was analyzed using the metaphase chromosome spread prepared from regenerative cells by an air-drying method. Results showed that the E.ignotus had a diploid chromosome number of 12 with a chromosome formula of 2n=12=10 m+2 sm, and its sex determining mechanism was the XX-XY type. There were ten autosomal biarmed chromosomes and two X chromosomes in females or one X and one Y in males. Five pairs including chromosomes 1-4, X chromosome and Y chromosome were metacentric chromosomes, while the remaining chromosome 5 was submetacentric. Every pair of chromosomes had their characteristic G-banding pattern. |
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