| 汪奇,郭正龙,李延伸,云丹,黄波,周忠良.2012.来曲唑对暗纹东方鲀性腺分化及相关基因表达的影响.动物学杂志,47(5):16-23. |
| 来曲唑对暗纹东方鲀性腺分化及相关基因表达的影响 |
| Effects of Letrozole on Gonadal Differentiation and Related Gene Expression in Gonad of Takifugu obscurus |
| 投稿时间：2012-02-28 修订日期：2012-04-18 |
| DOI： |
| 中文关键词: 性别分化 暗纹东方鲀 来曲唑 |
| 英文关键词:Sex differentiation Takifugu obscurus Letrozole |
| 基金项目:上海自然科学基金项目(No.08ZR1407300) |
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| 中文摘要: |
| 为了解雌激素在鱼类雌性性别分化中的作用,在克隆暗纹东方鲀(Takifugu obscurus)性腺CYP19A和DMRT1基因部分序列基础上,采用不同浓度芳香化酶抑制剂来曲唑 (letrozole, LE)(0、25、125、625、3125 μg/L)处理暗纹东方鲀初孵仔鱼,每个平行组各20尾鱼,观察CYP19A和DMRT1基因的表达情况和组织学变化。RT-PCR结果显示:25 μg/L LE实验组中样本CYP19A和DMRT1表达水平与对照组相比无显著差异;LE 125 μg/L以上各浓度组中约30%的样本同时表达CYP19A和DMRT1基因,且随着LE浓度增加,CYP19A表达量下调,而DMRT1表达量上调;组织学研究表明,125 μg/L LE以上各实验组样本中可见由雌性向雄性转变的间性体性腺。孵化后56d,125 μg/L LE实验组样本中约有20%显示雌性;625 μg/L LE实验组样本显示表型雄性和间性体,未见雌性;最高浓度3125μg/L LE组中所有样本均显示表型雄性,CYP19A和DMRT1基因表达与对照组雄性相同。上述结果说明,抑制内源雌激素合成可使暗纹东方鲀仔稚鱼CYP19A基因表达下调,同时DMRT1基因表达上调,并发生雄性化性逆转。 |
| 英文摘要: |
| In order to elucidate the role of estrogen in the female sex differentiation of Takifugu obscurus, partial sequences of CYP19A and DMRT1 cDNA in gonad of T.obscurus were cloned respectively. T.obscurus flies were exposed to 0, 25, 125, 625, 3 125 μg/L aromatase inhibitor letrozole (LE) to investigates the CYP19A and DMRT1 gene expression and histological changes. RT-PCR results showed that the CYP19A andDMRT1 expression level in 25 μg/L LE group did not differ from that of the control. In about 30% of the samples exposed to 125 μg/L or higher LE, both CYP19A and DMRT1 genes were expressed. With the increase of LE concentration, the CYP19A expression level decreased, while the DMRT1 expression level increased. Histologically, the samples treated with 125 μg/L or higher LE exhibited visible intersex gonads during their sex reversal from female to male. Fifty-six days after hatching, about 20% of the samples treated with 125 μg/L LE showed phenotypic female; the samples treated with 625 μg/L LE showed phenotypic male and intersex, without phenotypic female; all samples treated with 3 125 μg/L LE showed phenotypic male, and their CYP19A and DMRT1 expression levels were consistent with those of the control males. Conclusion: Inhibition of endogenous estrogen synthesis allows CYP19A expression downregulation, and DMRT1 expression upregulation inT.obscurus fly, and the occurrence of female-to-male reversal. |
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