| 张秀娟,李学家,季芳,靳丽莎,孙云霄,刘晓明,周彤,饶军华,彭白露.2012.高能量膳食对中老年食蟹猴糖尿病相关基因表达的影响.动物学杂志,47(6):112-118. |
| 高能量膳食对中老年食蟹猴糖尿病相关基因表达的影响 |
| Diabetes-associated Genes Expression after High Energy Diet in Middle-age and Aged Cynomolgus Monkeys |
| 投稿时间：2012-05-17 修订日期：2012-09-12 |
| DOI： |
| 中文关键词: 食蟹猴 2型糖尿病 外周血白细胞 基因表达 |
| 英文关键词:Cynomolgus Monkey (Macaca fascicularis) Type 2 diabetes mellitus (T2DM) Peripheral blood leucocytes Gene expression |
| 基金项目:国家"十二五"科技支撑计划研究项目(No.2011ZX09307-303-037),广州市重大科技专项项目(No.2010U1-E00811),广东省自然科学基金项目(No.10451026001004520) |
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| 中文摘要: |
| 本研究选择空腹血糖值(FPG)在正常范围内(3.20 mmol/L≤FPG<5.50 mmol/L)的中老年食蟹猴(Macaca fascicularis)60只,高能量膳食诱导12个月后,将其分为正常血糖组和诱高血糖组(FPG≥5.50 mmol/L)。采用荧光定量PCR技术对2组中36个糖尿病相关基因在诱导前后外周血白细胞中的mRNA表达量进行分析。结果表明,高能量膳食诱导后,诱高血糖组FPG和甘油三酯(TG)显著高于正常血糖组(P<0.05),而胆固醇(TCHO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)与分组无显著相关性(P>0.05)。基因表达水平上,诱高血糖组和正常血糖组均有血管紧张素转换酶基因(ACE)、肝糖原磷酸化酶基因(PYGL)、水通道蛋白基因(AQP2)等19个基因的mRNA表达量在高能量膳食诱导前后存在显著差异(P<0.05),且基因的表达模式变化一致,但诱高血糖组的mRNA表达量变化大,且三磷酸腺苷柠檬酸裂解酶(ACLY)、选择素L(SELL)、突触相关蛋白23(SNAP23)、突触融合蛋白(STX4)这4个基因的mRNA表达水平仅在诱高血糖组高能量膳食诱导前后呈差异表达(P<0.05)。 |
| 英文摘要: |
| A total of 60 middle-age and aged (above 10 years) Cynomolgus Monkeys (Macaca fascicularis) with normal fasting plasma glucose (FPG) values (3.20 mmol/L≤FPG<5.50 mmol/L) were fed by high energy diet for twelve months. After feeding, they were classified into two groups, normal FPG group and induced high FPG group (FPG≥5.50 mmol/L). Diabetes-associated gene expression profiles of peripheral blood leukocytes of the two groups were analyzed using quantitative real-time PCR before and after high energy diet, respectively. The results showed that FPG and triglyceride (TG) of induced high FPG group were strikingly higher than normal FPG group (P<0.05), but there was no significant difference in high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), cholesterol (TCHO) between the two feeding groups (P>0.05). Among 36 diabetes-associated genes tested, 19 genes (including angiotensin I converting enzyme (ACE), phosphorylase, glycogen, liver (PYGL), aquaporin 2 (AQP2)) displayed a similar expression pattern between induced high FPG group and normal FPG group and they were significantly up-regulated or down-regulated after high energy diet feeding (P<0.05), while expression variation of all these 19 genes were stronger in induced FPG group compared to normal FPG group. The expressions of ATP-citrate lyase (ACLY), selectin-L (SELL), synaptosomal-associated protein 23 (SNAP23) and syntaxin 4A (STX4) were significantly different only in induced high FPG group (P<0.05). |
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