| 张代臻,丁鸽,周婷婷,杨文娟,李佳,彭旭,唐伯平.2013.黄海海域口虾蛄种群的遗传多样性.动物学杂志,48(2):232-240. |
| 黄海海域口虾蛄种群的遗传多样性 |
| Genetic Diversity of Oratosquilla oratoria Populations in Yellow Sea |
| 投稿时间：2012-08-19 修订日期：2012-10-30 |
| DOI： |
| 中文关键词: 口虾蛄 黄海 COⅠ基因 遗传多样性 |
| 英文关键词:Oratosquilla oratoria Yellow Sea COⅠ gene Genetic diversity |
| 基金项目:国家自然科学基金项目(No.31000142,31071897);江苏省基础研究计划(自然科学基金)项目(No.BK2011421);江苏省高校自然科学基金重大项目(No.12KJA180009);盐城市农业科技指导性项目(No.YKN2011003);江苏省滩涂生物资源与环境保护重点建设实验室课题(No.JLCBE12002)和高层次人才项目(No.XKR2010003) |
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| 中文摘要: |
| 为了比较黄海海域口虾蛄(Oratosquilla oratoria)不同地理种群的遗传多样性水平,本研究通过分子标记探讨了6个口虾蛄种群的遗传变异。共获得72条627 bp的COⅠ序列,检测到41个变异位点,平均核苷酸多样性为0.005 12;定义了39个单元型,平均单元型多样性为0.962 5。6个种群间的遗传距离在0.004 3~0.006 4之间;盐城海域口虾蛄种群具有较高的遗传多样性水平,而青岛和日照海域种群的核苷酸多样性水平则相对较低。歧点分布和中性检验Tajima's D及Fu’s Fs值均说明黄海海域口虾蛄种群在历史上存在着一个快速扩张的事件,并且根据τ值和分子钟估计种群扩张时间大约距今5.5万年。本研究结果将为黄海海域口虾蛄野生资源的保护和合理利用提供科学依据。 |
| 英文摘要: |
| To reveal the genetic diversity of Oratosquilla oratoria from Yellow Sea, genetic variations among six populations were studied by molecular markers. A total of 39 haplotypes and 41 variable sites were defined in 627 sites for 72 COⅠ sequences. The average values of nucleotide diversity and haplotype diversity were 0.005 12 and 0.962 5, respectively. Genetic distance among populations ranged from 0.004 3 to 0.006 4. High genetic diversity was found in Yancheng population, but the lower nucleotide diversity was discovered in Qingdao and Rizhao populations. Results of mismatch distribution, Tajima’s D statistics (Tajima’s D=-2.051 69,P<0.05) and Fu’s F test (Fu’s Fs=-40.079) supported a sudden population expansion event. The time of expansion was estimated to occur about 55 000 years ago by τ value and molecular clock. The results could provide a scientific basis for protection and utilization of germplasm resources of O.oratoria in Yellow Sea. |
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