A simple and convenient method were introduced for making ecological specimens of small fishes. It can be achieved by steps as following: 1. preparation; 2. skinning; 3. to make and put carriage; 4. seaming; 5. prepare materials for filling; 6. to fill the materials; 7. to reshape and fit ocular prosthesis; 8. to dry, color, make ecological environments and fix. Compared to other methods, this new method is much easy, and it is convenient to control the amount of the filling materials, so that the specimens maken by this method is much more like the living status of the fish.