| 刘汝建,区又君,李加儿,苏慧,曹守花,王永翠.2013.盐度、温度对卵形鲳鲹选育群体肝抗氧化酶活力的影响.动物学杂志,48(3):428-436. |
| 盐度、温度对卵形鲳鲹选育群体肝抗氧化酶活力的影响 |
| Effects of Salinity and Temperature on the Activity of Antioxidant Enzymes in Livers of Selective Group of Trachinotus ovatus |
| 投稿时间：2012-12-07 修订日期：2013-03-18 |
| DOI： |
| 中文关键词: 卵形鲳鲹 盐度 温度 抗氧化酶 |
| 英文关键词:Trachinotus ovatus Salinity Temperature Antioxidant enzymes |
| 基金项目:国家"863"项目(No.2006AA10A414);中国水产科学研究院基本科研业务费项目(No.2012A0401,2013A0501) |
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| 中文摘要: |
| 通过盐度渐变和温度骤变的方法,分别研究了不同盐度(10、20、30、40)处理和不同温度(18.0℃、21.0℃、24.6℃、29.0℃、32.0℃)处理对卵形鲳鲹(Trachinotus ovatus)选育群体肝超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)活力的影响。在实验结束时,盐度10组酶活力与对照组无显著差异(P>0.05),盐度20组SOD活力极显著低于对照组(P<0.01);盐度40组120 h时,SOD和GPX活力极显著低于对照组(P<0.01),CAT活力与对照组差异不显著(P>0.05)。18.0℃和21.0℃ SOD活力在1、3、6、12、24 h这5个取样时间点均高于对照组,CAT活力在实验结束时(24 h)极显著高于对照组(P<0.01);29.0℃ SOD和CAT活力在实验结束时(24 h)显著高于对照组(P<0.05);32.0℃ SOD和CAT活力在5个取样时间点均显著低于对照组(P<0.05)。结果表明,适当的盐度或温度变化可以改变卵形鲳鲹肝抗氧化酶活力,达到机体耐受极限时酶活力下降。 |
| 英文摘要: |
| By changing water salinity gradually and temperature suddenly, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in livers of selective group of Trachinotus ovatus at different salinities (10, 20, 30, 40) and different temperatures (18.0℃, 21.0℃, 24.6℃, 29.0℃, 32.0℃) were studied respectively. At the end of the experiment, in groups with salinity of 10, the activity of enzymes had no significant difference with control groups (P>0.05); in groups with salinity of 20, the activity of SOD was significantly lower than that of control groups (P<0.01). In groups with salinity of 40, the SOD and GPX activities were significantly lower than those of control groups at 120 h (P<0.05), while the CAT activity had no significant difference compared with the control (P<0.05). In groups with temperatures of 18.0℃ and 21.0℃, the activity of SOD was higher than that of control groups at 1, 3, 6, 12, 24 h, while the activity of CAT was significantly higher than that of controls 24 h (P<0.01). At the end of the experiment (24 h),in groups with temperature of 29℃ the activities of SOD and CAT were significantly higher than those in control groups (P<0.05). In groups with temperature of 32.0℃, the activities of SOD and CAT were significantly lower than those of control groups at 5 sampling time points (P<0.05). The results indicate that the change of water salinity and temperature could enhances the antioxidant enzyme activity in T.ovatus liver. The activation can be inhibited when the salinity and temperature vary beyond the tolerance range of the body. |
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