| 张海琪,林静,黎飞,杜伟,王扬.2013.中华鳖日本品系体内氟苯尼考药物代谢动力学研究.动物学杂志,48(3):465-473. |
| 中华鳖日本品系体内氟苯尼考药物代谢动力学研究 |
| Pharmacokinetics of Florfenicol in Pelodiscus sinensis Japanese Strain |
| 投稿时间：2012-09-10 修订日期：2013-01-08 |
| DOI： |
| 中文关键词: 中华鳖日本品系 氟苯尼考 药动学 液相色谱-串联质谱 |
| 英文关键词:Pelodiscus sinensis Japanese strain Florfenicol Pharmacokinetics High-performance liquid chromatography-tandem mass spectrometry |
| 基金项目:浙江省科技厅重大攻关项目(No.2009C12075);浙江省重点标准化研究项目(No.2008-6) |
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| 中文摘要: |
| 应用液相色谱-串联质谱技术研究了氟苯尼考在中华鳖日本品系(Pelodiscus sinensis Japanese strain)体内的残留代谢规律。在(25.0±2.0)℃水温下,150只体重为(250±50) g健康中华鳖日本品系连续7 d投喂含30 mg/kg氟苯尼考的饲料,分别于最后一次给药后1 h、2 h、4 h、8 h、16 h、24 h、48 h、72 h、96 h、120 h、144 h、168 h、240 h、360 h采集肝、血液、肌肉和肾样品。样品中氟苯尼考的残留量采用乙酸乙酯提取,正己烷净化,电喷雾负离子多反应监测模式下进行测定,内标法定量,并利用3P97药动学软件进行数据分析。结果表明,氟苯尼考在中华鳖日本品系血液、肌肉、肝和肾中达峰浓度分别为167 μg/kg、188 μg/kg、67.15 μg/kg和85.71 μg/kg,达峰时间分别为4 h、8 h、8 h和8 h,消除半衰期分别为14.9 h、9.4 h、29.5 h和13.0 h,药动学方程分别为:C血液=210.332e-0.070 6 t+938.161e-0.046 5 t,C肌肉=5 642.635e-0.077 1 t+5 765.891e-0.074 4 t,C肝=111.596e-0.036 0 t+4.339e-0.023 5 t和 C肾=176.509e-0.065 4 t+615.697e-0.053 6 t。说明氟苯尼考在中华鳖日本品系体内残留代谢较快,建议休药期为15 d。 |
| 英文摘要: |
| Pharmacokinetics of florfenicol were studied in tissues from Pelodiscus sinensis Japanese strain using high performance liquid chromatography-tandem mass spectrometry. One hundred fifty healthy soft-shell turtles with the weight of 250±50 g were adnubustered florfenicol at a dosage of 30 mg/kg for 7 consecutive days under the temperature of 25.0±2.0℃. After the last feeding, tissues including blood, muscle, liver and kidney were collected at 1 h, 2 h, 4 h, 8 h, 16 h,24 h, 48 h, 72 h, 96 h, 120 h, 144 h, 168 h, 240 h and 360 h, respectively. Florfenicols in different tissues were extracted with ethyl acetate, cleaned by hexane, and detected by HPLC-MS/MS with negative electrospray ionization under multiple reaction monitoring. Quantitative analysis was used by internal standard method. Results showed that: The peak times of concentrations of florfenicol in plasma, muscle, liver and kidney were 4 h, 8 h, 8 h and 8 h, with the maximum content of 167 μg/kg, 188 μg/kg, 67.15 μg/kg and 85.71 μg/kg, respectively. The elimination half life was14.9 h, 9.4 h, 29.5 h and 13.0 h. The concentration-time curves of florfenicol in blood, liver, muscle and kidney were: Cblood=210.332 e-0.070 6 t +938.161 e-0.046 5 t, Cmuscle=5 642.635 e-0.077 1 t+5 765.891 e-0.074 4 t, Cliver=111.596 e-0.036 0 t+4.339 e-0.023 5 t and Ckidney=176.509 e-0.0654 t+615.697 e-0.053 6 t, respectively. Florfenicol was absorbed and eliminated fast in P.sinensis Japanese strain. The withdraw period should not be less than 15 d under this experiment condition. |
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