During the survey of cave bat in Henan Province, three specimens of Rhinolophus luctus were caught in the Water Cannel (N31°41', E115°04')-Hushan Reservoir, Shawo Town, Xinxian County, Xinyang City, the Taohua Cave (32°23'N, 113°16'E)-Tongbai Mountain, Tongbai County, Nanyang City, and the Longyuwan Mine (N33°42', E111°45')-Funiu Mountain, Luanchuan County, Luoyang City respectively from March to November, 2011. These specimens were identified as R.luctus lanous after measured the morphological parameters as well as those in other regions. This is the first time of the record of this species in Henan Province. The distribution of R. luctus in Henan Province is also discussed. Specimens are deposited in the herbarium of Henan normal university.