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闵金金,张加勇,郑荣泉,包得超.2013.跨种扩增筛选大鳄龟微卫星标记及遗传多样性分析.动物学杂志,48(6):926-932.
跨种扩增筛选大鳄龟微卫星标记及遗传多样性分析
Isolation of Microsatellite Markers by Cross-amplification and Genetic Diversity Analysis in Macroclemys temminckii
投稿时间:2013-01-21  修订日期:2013-05-15
DOI:
中文关键词:  大鳄龟  微卫星标记  跨种扩增  遗传多样性
英文关键词:Macroclemys temminckii  Microsatellite marker  Cross-amplification  Genetic diversity
基金项目:浙江省“十二五”水产育种重大专项(No.2012C12907-9),浙江省重点科技创新团队自主确定项目 (No.2010R50026-07),浙江省大学生科技创新活动计划资助项目(No.2012R404068),金华市科技局重点项目(No.2010-2-012 )
作者单位E-mail
闵金金 浙江师范大学生态研究所 金华 321004
浙江师范大学特种水产研究所 金华 321004 
 
张加勇 浙江师范大学生态研究所 金华 321004  
郑荣泉 浙江师范大学生态研究所 金华 321004
浙江师范大学特种水产研究所 金华 321004 
zhengrq@zjnu.cn 
包得超 浙江师范大学化学与生命科学学院 金华 321004  
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中文摘要:
      跨种扩增是一种能够快速、有效地获得物种微卫星标记的方法。本研究利用在近缘种中已发表的微卫星DNA引物,对大鳄龟(Macroclemys temminckii)进行跨种PCR扩增,在合成的69对引物中获得8对具有多态性的微卫星位点。对PCR扩增产物进行统计,得出观测杂合度(Ho)的范围是0.041 7~0.954 5,平均为0.384 8;期望杂合度(HE)的范围为0.041 7~0.811 8,平均为0.479 1;多态信息含量范围为0.040 0~0.759 2,平均为0.423 2;经过卡方检验后,部分微卫星位点符合哈迪-温伯格平衡。总体来说,这些位点是研究大鳄龟遗传结构的良好分子标记。
英文摘要:
      The method of cross-amplification can be used to obtain microsatellite marker quickly and effectively. This study amplified microsatellite loci of Macroclemys temminckii using published microsatellite DNA primers from related species. In M.temminckii, 8 microsatellite loci which had polymorphism were found in 69 microsatellite markers. By analyzing PCR amplification products, a total of 30 alleles were detected, and the average number of alleles was 3.75. The observed heterozygosity (Ho) ranged from 0.041 7 to 0.954 5, and expected heterozygosity (HE) was 0.041 7 to 0.811 8. PIC value was from 0.040 0 to 0.759 2, averaging 0.423 2. After chi-square test, some loci were found to correspond to Hardy-Weinberg equilibrium. In general, these loci are good molecular markers that can be used for studying genetic structure for M.temminckii.
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