Six forest bats(3♀,3♂)were collected from Nanling Nature Reserve in Guangdong Province in August 2012 and measurement was taken. The nostrils were short tubes; pelages were thick and soft; hind legs, wing membrane and uropatagium were covered with brown hair. Their sizes were relatively large and with obvious difference between male and female. The forearm length (FA) was 48.94-52.67 mm in female and 44.00-46.92 mm in male. The karyotype of this bat was 2n=44, FN=52. The echolocation calls were frequency modulated (FM) with main frequency 78.3 kHz. These individuals were identified as Harpiocephalus harpia (Murininae) based on the characteristics of external characteristics, skull morphologies and karyotypic information. The occurrence of this bat verified and confirmed their distribution in mainland China. This recording had a great significance in supplementing the distribution, behaviorl and ecology informations on this species.