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王峥,刘长琳,翟介明,林铃生,张盛农,陈四清,孙礼娟,葛建龙,边力.2021.莱氏拟乌贼缠卵腺的显微与超微结构.动物学杂志,56(1):80-87.
莱氏拟乌贼缠卵腺的显微与超微结构
Microstructure and Ultrastructure of the Nidamental Gland of Sepioteuthis lessoniana
投稿时间:2020-07-20  修订日期:2021-01-04
DOI:10.13859/j.cjz.202101010
中文关键词:  莱氏拟乌贼  缠卵腺  显微结构  超微结构
英文关键词:Sepioteuthis lessoniana  Nidamental gland  Ultrastructure  Microstructure
基金项目:中国水产科学研究院黄海水产研究所基本科研业务费(No. 20603022020018),现代农业产业技术体系项目(No. CARS-49),烟台市重点研发计划项目(No. 2017YT06830785)
作者单位E-mail
王峥 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ③ 上海海洋大学水产与生命学院 上海 201306 1214864423@qq.com 
刘长琳 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ② 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 liuchl@ysfri.ac.cn 
翟介明 ④ 莱州明波水产有限公司 莱州 261418 mbaquatic@163.com 
林铃生 ⑤ 福建省逸有水产科技有限公司 漳州 363402 lls998997996@163.com 
张盛农 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ② 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 zhangsn@ysfri.ac.cn 
陈四清 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ② 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 chensq@ysfri.ac.cn 
孙礼娟 ④ 莱州明波水产有限公司 莱州 261418 mbaquatic@163.com 
葛建龙 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ② 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 gejl@ysfri.ac.cn 
边力 ① 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所 青岛 266071 ② 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室 青岛 266071 bianli@ysfri.ac.cn 
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中文摘要:
      为了解莱氏拟乌贼(Sepioteuthis lessoniana)缠卵腺的结构和功能,本研究采用组织切片技术和透射电镜技术对该腺体进行显微与超微结构观察。结果显示,缠卵腺由腺壁组织、分泌叶瓣和结缔组织组成。其中,腺壁组织由外膜层和肌肉层组成,位于腺体外部;分泌叶瓣是腺体的主要部分,由分泌细胞和支持细胞组成,分泌细胞具有分泌功能,支持细胞起到支持分泌细胞的作用。分泌叶瓣两侧为分泌细胞,分泌细胞较大,细胞内细胞器丰富,包括大量线粒体、内质网和高尔基体,此外分泌细胞内充满分泌物质,主要是蛋白泡。结缔组织夹杂在外膜层和分泌叶瓣中。分析认为,莱氏拟乌贼的缠卵腺是一种典型的分泌型腺体,其分泌的凝胶物质主要功能是形成第三层卵膜,以保护受精卵免受外界环境侵袭。
英文摘要:
      In natural waters, the spawning reefs of Sepioteuthis lessoniana are mostly distributed in small rocks, broken shells and seaweed clumps, which interact with environmental pollutants. Therefore, certain deep studies have become extremely important to obtain insights into the adaptation of its structure and function to sophisticated environment during the breeding. Herein, the histological structure and cell type of the nidamental gland of adult S. lessoniana were observed by tissue slice techniques and transmission electron microscopy. The results showed that the surface of the nidamental gland was smooth and milky, with parallel lines on the surface (Fig. 1). and the nidamental gland was composed of three subdivisions: glandular wall tissue, secretory lobe and connective tissue. The glandular wall tissue was composed of the outer membrane layer and the muscular layer, which was located outside of the gland (Fig. 2a). The secretory lobe was the most important structure in gland and was layered (Fig. 2d). Connective tissue mainly existed in the junction of glandular wall tissue and secretory lobe, basement membrane of secretory lobe, and extracellular matrix between secretory cells (Fig. 2a﹣c). Further, the cell composition of the secretory lobes was dissected. The secretory lobes comprised two cell types, supporting cells and secretory cells (Fig. 3a). The supporting cells were small, oval in shape, located at the base of the secretory lobe, and the organelles in the cells were rare, and only a few mitochondria were observed (Fig. 3a, c). However, the secretory cells were large and rectangular with a large number of organelles, including mitochondria, endoplasmic reticulum and Golgi complex, visible in the cells (Fig. 3a, d, e). And we observed surprisingly few vesicles and protein vesicles, which were likely concentrated on the outside of the cell. Together, cilia were abundantly produced (Fig. 3b, f). We conclude that the nidamental gland is a typical secretory gland, and that primary function of the gel-forming substances is to form the third layer, which plays an essential role in protecting the fertilized egg against various environmental pollutants.
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