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魏艳慧,蒋一婷,毕雨佳,万冬梅,王娟.2021.不同性别杂色山雀血浆活性氧和超 氧化物歧化酶与繁殖成效的关系.动物学杂志,56(3):399-404.
不同性别杂色山雀血浆活性氧和超 氧化物歧化酶与繁殖成效的关系
The Relationships of Reproductive Success with Different Genders’ Plasma Reactive Oxygen Species and Superoxide Dismutase in the Varied Tit (Sittiparus varius)
投稿时间:2020-09-07  修订日期:2021-05-08
DOI:10.13859/j.cjz.202103010
中文关键词:  氧化应激  活性氧  超氧化物歧化酶  繁殖成效  杂色山雀
英文关键词:Oxidative stress  Reactive oxygen species  Superoxide dismutase  Reproductive success  Varied Tit, Sittiparus varius
基金项目:国家自然科学基金项目(No. 31872231),辽宁大学青年科研基金项目(No. LDQN2019012)
作者单位E-mail
魏艳慧 辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室 沈阳 110036 404605396@qq.com 
蒋一婷 辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室 沈阳 110036 yiting.jiang@lnu.edu.cn 
毕雨佳 辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室 沈阳 110036 614839416@qq.com 
万冬梅 辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室 沈阳 110036 wdm9610@163.com 
王娟 辽宁大学生命科学院辽宁省动物资源与疫病防治重点实验室 沈阳 110036 wangjuan@lnu.edu.cn 
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中文摘要:
      氧化应激是指活性氧等氧化剂的产生大于抗氧化防御系统清除能力时的不平衡状态,是个体应对内外环境刺激的适应性生理机制,是衡量个体身体状况的综合性生理指标。为探究氧化应激对鸟类繁殖的预测作用,本研究测量了育雏前期(雏鸟6 ~ 8日龄)杂色山雀(Sittiparus varius)亲鸟血浆氧化应激分子活性氧和超氧化物歧化酶含量,通过巢箱监测获得了杂色山雀的繁殖参数,采用偏最小二乘回归法分析杂色山雀氧化应激对其雏鸟出飞率和繁殖成功率的预测作用。结果显示,雌性杂色山雀亲鸟血浆活性氧浓度与雏鸟出飞率(n = 13,P < 0.05)、繁殖成功率(n = 13,P < 0.01)均呈显著负相关关系,即血浆活性氧水平越低的雌性杂色山雀,其繁殖巢雏鸟出飞率和繁殖成功率越高;雌性杂色山雀亲鸟血浆超氧化物歧化酶浓度与雏鸟出飞率(n = 13,P > 0.05)、繁殖成功率(n = 13,P > 0.05)无显著相关关系。本研究中这两项血浆氧化应激标记物与杂色山雀雄性亲鸟繁殖成效间均无显著的相关关系。该结果表明,杂色山雀雌性亲鸟活性氧水平对其繁殖成效具有显著的预测作用。
英文摘要:
      The difference in bird's body condition may lead to the variation of reproductive success. Recent works have suggested that biomarkers of oxidative stress might provide an additional tool to assess the health state of individuals. Oxidative stress is usually defined as the imbalance between pro-and anti-oxidative processes. Oxidative stress is the physiological adaptation mechanism of individuals to cope with stress, that reflects the environmental conditions that birds experience. The oxidative status also a priori informative tool to evaluate the reproduction and survival of individuals. However, the conclusion about the role of oxidative stress on the reproductive of wild organisms is not consistent. In this study, to explore whether the plasma biomarkers of oxidative stress can predict the reproductive success of Varied Tit (Sittiparus varius), we took blood samples from the pairs of Varied Tit bred in the artificial nest boxes during their early brooding period (6﹣8 days post-hatching). Then we used the ELISA kit to measure the levels of plasma oxidative stress (reactive oxygen species, ROS and superoxide dismutase, SOD concentration). We also got the reproductive parameters of Varied Tit by nest monitoring. Partial least squares regression (PLS) analysis was used to analyze the relationship between oxidative stress and reproductive success (fledging rate and breeding success). We found that there are no significant differences between males and females in plasma reactive oxygen species and plasma superoxide dismutase concentration during chick-rearing period (independent- samples t-test: reactive oxygen species, t (20, 25) =﹣0.887, P = 0.380; superoxide dismutase, t(20, 25) =﹣1.325, P = 0.192). We also found that the plasma reactive oxygen species concentration was significantly negatively correlated with the fledging rate (partial least squares regression: B =﹣0.966, P = 0.013, Fig. 1 a) and breeding success (partial least squares regression: B =﹣0.976, P = 0.002, Fig. 2 a) of female Varied Tits, and females with a lower reactive oxygen species concentration tended to have a higher fledgling rate and breeding success. The reproductive success of female Varied Tit was not related to the plasma superoxide dismutase concentration, and there was no statistical significance in any analysis of males. Our findings suggest that reactive oxygen species, as a biomarker of oxidative stress in plasma, could be a tool to assess the fitness components and predict the reproductive success of female Varied Tits.
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