[Objectives] Positive reinforcement blood collection training allows to collect blood samples from animals without physical or chemical bonding, minimizing injuries due to stress. In conventional positive reinforcement blood collection training, non-fixed reinforcement, mainly food, is used as a reinforcer to maintain the movement. In this study, after failing to train three chimpanzees in blood collection using non-fixed reinforcement, music was added to the blood collection training as a continuous positive reinforce and timer for the first time, based on the characteristic that chimpanzees love music and can recognize musical melodies. [Methods] The retention time of blood collection action of each chimpanzee during training without music and with music was recorded respectively. SPSS Statistics (version 20.0) was used for statistical analysis. First, Shapior-Wilk test was used for normality test. For data conforming to normal distribution, paired sample t-test was used for testing. Conversely, Wilcoxon signed-rank test was used for data that are not normally distributed. [Results] When using music for blood collection training, the movement retention time of the three chimpanzees was significantly higher than that of the non-fixed reinforcement method (without music) (Table 1). The number of times each chimpanzee completed the training, that is, the minimun number of times required to achieve the required training effect varies. Beibei had the most training sessions (177 times) and the training difficulty was sharp needle desensitization for 4 min, followed by Yaya (69 times) and the training difficulty was holding the movement for 4 min. Mingming had the fewest training sessions (42 times) (Table 2). [Conclusion] Upon completion of training, all three chimpanzees were able to complete blood collection during 4 min of music playing, without feeding any food in the middle of the session. That is, music can prolong the movement retention time of chimpanzees during blood collection training and increase the stability of training. The methods and study can provide a sound reference and approach for related workers in the same industry.