During the investigation on amphibians in October 2022, 5 female specimens of Rana (Anura:Ranidae) were collected from Xianliang Town, Qingyuan County, Zhejiang Province, China. We compared their features by morphology and phylogeny and identified them as R. longicrus. This represents the first record of this species in Zhejiang Province. 5 female samples collected from Qingyuan County were measured with electronic vernier calipers. The measurements were:snout-vent length, head length, head width, snout length, internasal space, interorbital space, diameter of eye, length of lower arm and hand, tibia length, foot length, and hindlimb length. We compared these morphological characteristics with the specimens from Fujian, Jiangxi, Guangdong, and Taiwan, China. In this study, phenol/chloroform extraction was used to extract genomic DNA from the hindlimb muscle tissue of Brown Frogs collected in Qingyuan County, Zhejiang Province. 16S rRNA gene was amplified, and the primers were 5′-CGC CTG TTT ACC AAA AAC AT-3′ and 5′-CCG GTC TGA ACT CAG ATC ACG T-3′. Based on 16S rRNA gene fragments, we used Mrbayes-3.1.2 to construct Bayesian tree for phylogenetic analysis and used p-distance in MEGA 7 to calculate the genetic distance between species of Rana. All the new sequences in this research have been uploaded to the GenBank database. The 16S rRNA sequences of 16 species of Rana distributed in East China and Glandirana tientaiensis were obtained from GenBank (Table 2).The morphological characteristics are consistent between the specimens collected in this study and the topotype of R. longicrus. Our specimens have a medium snout-vent length (40.4 to 55.2 mm); head length is larger than the head width; some sparse granules can form a V-shaped figure on the shoulder; dorsolateral fold obvious and connect with the crotch; arms and legs with dark brown bands; tibiotarsal articulation beyond snout. In addition, the Bayesian analyses based on 16S rRNA strongly support the specimens we collected form a monophyletic clade with the topotype specimens (Fig 2), and the genetic distance is less than 0.005% between our specimens and topotype (Table 3).The specimens collected in Qingyuan County are determined to be R. longicrus, which is the first record of this species in Zhejiang Province. In this study, we further updated the distribution range of R. longicrus.