| 马琦,黄静,万里鹏,侯明,袁智勇,王志坚.2023.重庆彭水发现务川臭蛙.动物学杂志,58(4):622-629. |
| 重庆彭水发现务川臭蛙 |
| Odorrana wuchuanensis Found in Pengshui, Chongqing |
| 投稿时间：2022-11-26 |
| DOI：10.13859/j.cjz.202304015 |
| 中文关键词: 两栖动物 臭蛙属 彭水苗族土家族自治县 物种保护 |
| 英文关键词:Amphibians Odorrana Pengshui Miao and Tujia Autonomous County Species protection |
| 基金项目:重庆市国家重点保护陆生野生动物补充调查项目(No. sxxycq-2021-086)； |
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| 中文摘要: |
| 2022年7月，在重庆市彭水苗族土家族自治县新田镇调查到2只臭蛙，经比较形态特征确认为臭蛙属(Odorrana)物种。基于线粒体16S rRNA基因片段构建的臭蛙属部分物种的贝叶斯和最大似然系统发育树显示，这2只臭蛙和模式产地贵州省务川仡佬族苗族自治县的务川臭蛙(O. wuchuanensis)聚为一支，具有较高的支持率，贝叶斯后验概率为0.99、最大似然超快速引导支持(2 000次重复)自展值为95%。综合形态特征和系统发育分析，确认此2只臭蛙为务川臭蛙，系重庆市首次记录。务川臭蛙分布范围较为狭窄，此前仅记录于贵州省务川县、沿河土家族自治县、荔波县以及湖北省建始县和广西壮族自治区环江毛南族自治县。务川臭蛙在重庆彭水的发现，确认了该物种在武陵山和大娄山脉重庆段的分布。彭水发现的务川臭蛙1雄1雌，雄性体长81.3 mm，大于模式标本的最大体长，大于湖北建始县种群雄蛙体长；雌性体长87.1 mm，在模式标本的体长范围内，小于建始县种群雌蛙体长。线粒体16S rRNA基因的遗传分化分析显示，重庆市彭水苗族土家族自治县的务川臭蛙样本和模式产地的样本没有遗传分化，遗传距离为0。务川臭蛙在重庆市的发现扩展了对该种分布区的认知，并可为物种保护研究提供基础信息。 |
| 英文摘要: |
| During a field investigation in Xintian Town, Pengshui Miao, and Tujia Autonomous County, Chongqing in July 2022, two Odorrana specimens were found (Fig. 1). The purpose of this study is to identify the species using morphological and phylogenetic methods, as well as to ascertain its distribution in Chongqing.A comparative analysis of the morphological characteristics of the species discovered from Chongqing and O. wuchuanensis from Guizhou and Hubei was conducted. For phylogenetic analyses, mitochondrial 16S rRNA fragments were amplified, and the 16S rRNA gene information of 11 Odorrana species from China and abroad was obtained from the GenBank database (Table 1). Corresponding sequences of one Pseudorana weiningensis and one Nidirana daunchina were also downloaded and used as outgroups. The maximum likelihood phylogenetic tree was constructed in IQ-TREE, the Bayesian tree construction was carried out in MrBayes 3.2.1, and the best-fit model was selected according to the Bayesian inference criteria (BIC) computed with JModelTest 0.1.1. Morphological characteristics appear to differ little between those specimens of Chongqing, Guizhou, and Hubei (Fig. 2). The snout vent length of the male species discovered in Pengshui was 81.3 mm, which was longer than the maximum snout vent length of the type specimen and O. wuchuanensis from Jianshi County, Hubei Province. The female of the species had a snout vent length of 87.1 mm, which was within the range of the type specimen’s body snout vent length but smaller than the female O. wuchuanensis from the Jianshi County (Table 2). Genetic analysis based on the mitochondrial 16S rRNA gene revealed that the specimens found in Pengshui County of Chongqing clustered with O. wuchuanensis from Wuchuan County of Guizhou Province with a high support (Fig. 3). The genetic distance between O. wuchuanensis in Wuchuan County, Guizhou Province, and the specimens found in Pengshui County is 0 (Table 3). According to a thorough comparison with morphological analysis and phylogenetic analysis, the specimens discovered in Pengshui were identified as O. wuchuanensis, which is a new record of amphibian distribution in Chongqing. |
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