[Objectives] Behavioral lateralization is widespread in different animal taxa. Studies have shown that differences in behavioral responses to specific stimuli between left- and right-lateralized individuals may have fitness consequences. This suggests that behavioral lateralization may have significant impact on animals’ adaptation to the natural environment. However, current studies on fitness-related behaviors of lateralized individuals mainly focus on captive animals or wild-caught animals in laboratory environment, and few studies have compared the adaptive differences between left- and right-lateralized individuals under natural conditions. Hence, the understanding of differences in response behaviors to stimuli in a natural setting between left- and right-lateralized individuals remains limited. In this study, we investigated the relationship between behavioral lateralization (specifically, footedness) and nest-site choice in response to human infrastructures in a wild population of Japanese Tits Parus minor in a rural area near the Dongzhai National Nature Reserve, Henan Province, China. [Methods] During the breeding season of Japanese Tits in 2022, we monitored their choice of nest boxes, and caught parental birds and tested their foot-use preference while feeding meal worms. Based on 77 individuals (including 45 males and 32 females) with footedness data available, we conducted generalized linear models with a Gamma distribution and a log link to compare the distances from the nest site to the nearest road and the nearest building, respectively, between left- and right- footed birds. Three explanatory variables (i.e. individual footedness, relative clutch initiation date and the interaction of above two terms) were included in the modeling process. [Results] The results showed that there was no significant difference in the distances from the nest site to the nearest road and the nearest building between left- and right-footed males (Table 1). For females, although the distances from the nest sites to the nearest road increased with clutch initiation date for both left- and right-footed individuals, the distances from the nest sites to the nearest building increased with clutch initiation date in left-footed individuals but remained relatively constant in right-footed individuals (Table 1, Fig 1). [Conclusion] The results suggest a relationship between behavioral laterality of animals and their responses to human disturbance in the wild. Given inadequate study on this topic, we suggest that more work should be done in wild animals for a better understanding of behavioral differences of differently lateralized individuals in their natural environments and the influence of behavioral lateralization on their ability to adapt to environments.