Abstract:[Objectives] From 2019 to 2023, 2 specimens of the genus Eothenomys were collected from Gong County (104°50′05″ E, 27°54′27″ N, 1 331 m) and 4 from Xuyong County (105°32′05″ E, 28°13′51″ N, 1 370 m), both in southern Sichuan. These specimens were tentatively identified as Eothenomys eleusis, but their species status is controversial. Recently, E. eleusis has been confirmed as an independent species by morphological and molecular phylogenetics studies. Current research has shown that there are errors in the identification of E. eleusis distributed in Shimian, Meigu, Muli, and other places in Sichuan. In this study, we aim to identify these 6 specimens to update the records of the distribution range of E. eleusis. [Methods] The specimens (voucher numbers:SAF19843, SAF19844, SAF220125, SAF220131, SAF23176, and SAF23177) are stored at the Animal Herbarium of Sichuan Academy of Forestry. We measured the external measurement and skull measurement in 3 of these specimens and compared them with type specimens. Meanwhile, we sequenced the Cyt b gene in 4 of these specimens, and estimated Kimura 2-parameter (K2P) genetic distance using these sequences. In addition, we used the Cyt b gene sequences to construct a Bayesian phylogeny tree of the genus Eothenomys. [Results] According to the morphological description (Fig. 1) and comparison (Table 1) of 3 specimens collected in Sichuan, their tail lengths were about 41% of their body lengths, and the total length of cranium was less than 26.50 mm, which was consistent with that of the E. eleusis. In the phylogeny tree of the genus Eothenomys, the specimens collected in Sichuan and E. e. eleusis was the first cluster, which with E. e. aurora was the second cluster, and then with E. miletus to be the third cluster. The K2P genetic distance between the samples from Sichuan and E. e. eleusis is 0.8%, while between the samples from Sichuan and E. e. aurora is 2.5%. [Conclusion] The classification of E. eleusis is discussed in this paper and confirms the distribution of the E. eleusis in Sichuan. The morphological characteristics of E. eleusis in Sichuan are consistent with those of E. e. aurora, but their genetic relationship is closer to E. e. eleusis in terms of molecular genetic information, which indicate the differentiation of subspecies cannot be reflected in the genetic information of a single gene or several genes, and this discovery provides a reference for species classification research and zoogeography research.