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杨纯,师婷婷,王新月,寇曌婷,孙金玉.2024.秦岭滑蜥断尾再生伤口愈合阶段细胞因子的时空分布特征.动物学杂志,59(2):245-257.
秦岭滑蜥断尾再生伤口愈合阶段细胞因子的时空分布特征
The Spatial-Temporal Distribution of Cytokines in the Wound Healing Stage of Regenerating Tail in Scincella tsinlingensis
投稿时间:2023-05-08  
DOI:10.13859/j.cjz.202423087
中文关键词:  秦岭滑蜥  断尾再生  伤口愈合  细胞因子
英文关键词:Scincella tsinlingensis  Tail regeneration  Wound healing  Cytokines
基金项目:山西省基础研究计划自由探索类项目(No. 20210302123323),山西师范大学自然科学基金项目(No. 020820220007);
作者单位
杨纯 山西师范大学生命科学学院 太原 030031 
师婷婷 山西师范大学生命科学学院 太原 030031 
王新月 山西师范大学生命科学学院 太原 030031 
寇曌婷 山西师范大学生命科学学院 太原 030031 
孙金玉 山西师范大学生命科学学院 太原 030031 
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中文摘要:
      细胞因子参与免疫应答、介导炎症反应,进而在损伤组织的再生修复过程中发挥调控作用。通过切割法建立秦岭滑蜥(Scincella tsinlingensis)断尾再生模型,针对伤口愈合阶段,采用免疫组织化学方法检测促炎症因子白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-1β(IL-1β)、白细胞介素-1受体1(IL-1R1)、肿瘤坏死因子-α(TNF-α)和抗炎细胞因子白细胞介素-10(IL-10)的组织细胞定位及表达变化。结果显示:6种细胞因子在断尾脊髓和椎骨中的骨系细胞中均呈阳性。断尾创面组织及其附近鳞片下方真皮层中IL-6阳性细胞数量先增加后减少,断尾后3 d达峰值194.3 ± 24.9。断尾后3 d,创面IL-8阳性细胞数目为107.7 ± 12.7,超过真皮层的43.7 ± 9.1。IL-1β和IL-1R1阳性细胞数量先增后减,伤口部位的阳性细胞明显多于真皮层(P < 0.05)。TNF-α阳性细胞由0.5 d的126.3 ± 35.0增至3 d的190.3 ± 12.1。IL-10阳性细胞断尾后总体呈现先增后减的趋势,断尾后0.5 d伤口部位阳性细胞达201 ± 17.8,显著高于原尾(P < 0.05)。上述结果表明,促炎症因子IL-6、IL-8、IL-1β及其受体IL-1R1、TNF-α和抗炎细胞因子IL-10与创面髓系来源细胞的空间分布相一致,共同参与秦岭滑蜥断尾伤口愈合,并可能通过维持免疫抑制的微环境实现无疤痕伤口愈合,以促进芽基形成。
英文摘要:
      [Objectives] Cytokines participate in the immune response, mediate the inflammatory response, and then play a regulatory role in the regeneration and repair process of damaged tissues. [Methods] The tail of Scincella tsinlingensis was amputated with a razor blade to set up tail regeneration model, and immunohistochemical methods were used to detect the histologic localization and expression changes of cytokines interleukin, IL-6, 8, 1β (IL-6, IL-8, IL-1β), interleukin 1 receptor type 1 (IL-1R1), tumour necrosis factor-α (TNF-α) and interleukin, IL-10 (IL-10) at the stage of wound healing. Three visual fields of different parts were randomly selected under microscope with 400-fold magnification for immunopositive cell count. The results were expressed as “Mean ± SD”, one-way ANOVA and Tukey HSD were used to analyze the difference in the number of positive cells on different days. [Results] The results showed that six cytokines were positive in bone cells in the spinal cord and vertebrae. The number of IL-6 positive cells in the wound site and the dermis of proximal scale to the stump surface initially showed increasing and later decreasing of these cells, reaching a peak of 194.3 ± 24.9 at 3 d after tail amputation, the number of IL-8 positive cells in the wound surface was 107.7 ± 12.7, exceeding 43.7 ± 9.1 in the dermis at 3 d after tail amputation. The number of IL-1β and IL-1R1 positive cells initially showed increasing and later decreasing of these cells, and significantly more positive cells occurred in the wound site than in the dermis (P < 0.05). The TNF-α positive cells increased from 126.3 ± 35.0 at 0.5 d to 190.3 ± 12.1 at 3 d. The number of anti-inflammatory cytokine IL-10 positive cells initially showed increasing and later decreasing of these cells after tail amputation. At 0.5 d after tail amputation, positive cells reached 201 ± 17.8, which is significantly higher than that in the original tail (P < 0.05, Fig. 7). [Conclusion] The above results indicate that IL-6, IL-8, IL-1β, IL-1R1, TNF-α and IL-10 are consistent with the spatial distribution of myeloid-derived cells, jointly participate in tail wound healing of S. tsinlingensis, and may achieve scarless wound healing and promote blastema formation by maintaining an immuno-suppressive microenvironment.
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