One small-sized bat specimen was collected on 23rd, November, 2019 from Baiyun mountain forest park, Lishui, Zhejiang (28°29′51″ N, 119°54′34″ E, 306 m). The main characteristics were diagnosed as follows: The forearm length was 35.4 mm; the ear conch was semi-transparent, rounded and funnel-shaped; the caudal vertebras were absent; the interfemoral membrane was poorly developed and in “∧” shape; the color of pelage was blackish brown on the base and russet on the tip; the wing membrane was dark-brown (Fig. 1). Based on morphological (Table 1) and molecular (Fig. 3) data, this specimen was identified as Tail-less Leaf-nosed Bat (Coelops frithii), which is the first record in Zhejiang Province. In addition, CT scanning and computer-assisted 3D-image reconstruction techniques were utilized to scan and reconstruct the skull of this specimen (Fig. 2). With these two non-destructive sampling techniques, osteological structures and features were obtained, while the skull remains intact. New digital approach was demostrated for osteological description and biological study in small mammals.