[Objectives] The Silver Pheasant (Lophura nycthemera whiteheadi) and Red Junglefowl (Gallus gallus jabouillei) are two Galliformes birds distributed in the Wuzhishan of Hainan Province. The present study aims to reveal the effects of habitat factors on the distribution of these two sympatric birds. [Methods] We set up 50 infrared cameras and obtained data from September 2018 to September 2020 in the northeast of Wuzhishan of Hainan Tropical Rainforest National Park (see Fig. 1). The chi-square goodness of fit test was used to analyze the influence of the number of infrared camera monitoring points on the observation value, Mann-Whitney U test was used to analyze the differences in resource utilization between species and within different seasons, and Friedman test was used to analyze the difference of vegetation resource utilization among species. Niche breadth and niche overlap were calculated using "shannon" and "morisita" of the Spaa package in R 4.1.1. [Results] The results showed that there were significant differences in the utilization of various types of vegetation resources between two species in different seasons (c2 = 76.324, df = 4, P < 0.05). The Silver Pheasant preferred tropical lowland rainforest and tropical montane rainforest, while the Red Junglefowl preferred secondary forest and tropical lowland rainforest (Table 1). There were significant differences in altitude distribution between the two species in dry season (U = 11 507.00, Z =﹣4.588, P < 0.05) and rainy season (U = 15 199,00, Z =﹣7.739, P < 0.05). The Red Junglefowl had a single peak altitude distribution, with a peak of 575.8 m in the dry season and 497.7 m in the rainy season and preferred sunny slopes (Fig. 2). In dry season, the slope distribution of the two species was significantly different (U = 12 414.00, Z =﹣3.743, P < 0.05). There was no significant difference in slope distribution between the two species in rainy season (U = 26 030.00, Z =﹣0.556, P > 0.05). The peak slopes of the Silver Pheasant were 19.7° and 34.5° in the dry season, and the peaks slopes were 20.1° and 34.3° in the rainy season. As to the Red Junglefowl, there was a single peak in the dry season of 35.7°, with double peaks in the rainy season of 12.5° and 35.0° (Fig. 4). During dry season, there was no significant difference in the aspect distribution of the two species (U = 15 678.50, Z =﹣0.688, P > 0.05). In the rainy season, the aspect distribution of the two species was significantly different (U = 22 060.00, Z =﹣3.296, P < 0.05), the Silver Pheasant was evenly distributed in the slope direction, and the Red Junglefowl mainly moved on the sunny slope (Fig. 5). There was significant difference in temperature selection of the two species in dry season (U = 12 088.00, Z =﹣4.042, P < 0.05) and rainy season (U = 19 478.50, Z=﹣4.900, P < 0.05). The temperature selections of the two species were both unimodal, with peaks both at 20.3 ℃ in dry season, and 22.2 ℃ of Silver Pheasant, 26.1 ℃ of Red Junglefowl in rainy season (Fig. 3). The niche overlap of the two species at altitude was the lowest (0.375 in the dry season and 0.353 in the rainy season), and being higher in temperature and slope (Table 2). [Conclusion] Our results indicated that the environmental adaptability of Silver Pheasant being better than that of Red Junglefowl. Niche differentiation of Silver Pheasant and Red Junglefowl can be realized mainly through the choice of altitude. There are also different degrees of differentiation characteristics in other environmental factors.