| 刘真诚,谷思雨,缪炜,巴桑,姜传奇.2022.西藏三种苔藓栖生纤毛虫的形态和系统发育分析.动物学杂志,57(2):277-288. |
| 西藏三种苔藓栖生纤毛虫的形态和系统发育分析 |
| Morphological and Phylogenetic Studies on Three Moss Dwelling Ciliates in Tibet |
| 投稿时间：2021-10-11 修订日期：2022-03-08 |
| DOI：10.13859/j.cjz.202202013 |
| 中文关键词: 西藏 苔藓 纤毛虫 形态 系统发育 |
| 英文关键词:Tibet Moss Ciliate Morphology Phylogeny |
| 基金项目:第二次青藏高原综合科学考察研究项目（No. QZKK0403），国家自然科学基金项目（No. 32070418） |
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| 中文摘要: |
| 本研究利用活体观察和蛋白银染色方法对采自西藏日喀则和那曲的3种苔藓栖生纤毛虫，鬃异源棘尾虫（Tetmemena pustulata）、棘毛虫未定种（Sterkiella sp.）和殖口虫未定种（Gonostomum sp.）进行了形态学研究，描述了活体、核器以及纤毛图式等形态学特征。同时，测定了这3种纤毛虫的SSU rDNA基因序列，并基于该序列进行了系统发育分析。3种纤毛虫首次在西藏苔藓生境发现，该研究丰富了对纤毛虫物种多样性和生态分布的认知，为纤毛虫原生动物生物地理学研究提供基础资料，同时也获得了这3种西藏苔藓栖生纤毛虫的种质资源，为纤毛虫种质和遗传资源的挖掘和利用提供基础。 |
| 英文摘要: |
| [Objectives] Three species of moss dwelling ciliates (3 genera, 2 families) were isolated from moss in Tibet and were identified by using morphological study. [Methods] The ciliates were studied morphologically using living observation and protargol staining methods. Morphological characters of three ciliates were counted for comparison with similar species, and phylogenetic trees were constructed for molecular identification. [Results] The morphological characteristics of Tetmemena pustulata (Fig. 1), Sterkiella sp. (Fig. 2) and Gonostomum sp. (Fig. 3) were described, respectively. The morphological characters of the Tibetan population of Tetmemena pustulata remain consistent with previous descriptions, but both Sterkiella sp. and Gonostomum sp. differ from known species. The SSU rDNA gene sequences of the three ciliates were amplified and phylogenetic analysis was conducted based on these sequences (Fig. 4). These three moss dwelling ciliates in this study were found in Tibet for the first time. The results of the phylogenetic analysis showed that the Tibetan population of T. pustulata clustered with the sequence of this species sequences from GenBank, the Tibetan population of Sterkiella sp. clustered with Sterkiella paratricirrata (MN191544), and the Tibetan population of Gonostomum sp. with G. sinicum (KY475614) and Gonostomum sp. (MG603605) clustered together as one branch, but by morphological comparison, the Tibetan population of Sterkiella sp. and S. paratricirrata showed obvious different characters of morphology, and the morphological characters of Tibetan population of Gonostomum sp. also differed significantly from those of G. sinicum. [Conclusion] Based on morphological studies and phylogenetic analysis, these three ciliates are finally identified as T. pustulata, Sterkiella sp. and Gonostomum sp. This study enriches the species diversity and ecological distribution of ciliates, providing further information of ciliate protozoans biogeography. Furthermore, this study obtains three moss ciliate resources, which will provide basis for mining and usage of ciliates germplasm and genetic resources. |
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