[Objectives] Bats are widely distributed around the world except the polar region. With the expansion of the sphere of human activity, the risk of human-bat contact is increasing. It is a common phenomenon in life that bats flying into human houses, however, there is no relevant research on proposing a method to deal with the conflict. [Methods] In this study, Asian Particoloured Bats (Vespertilio sinensis) were collected from May to July 2021 in Sijia Township of Changchun City, Jilin Province, and simulated experiments were conducted in a field laboratory. Three interference factors were set up in this experiment - artificial repel, light and noise. These factors were combined into eight interference patterns, including no interference, only light, light + repel, only repel, only noise, repel + noise, light + noise, repel + light + noise. Seven experiments were performed in each pattern, with an individual bat being put into the field laboratory. We recorded the time of the bats flew out the house and implied R.4.0.4. to carry out the Bonferroni multiple comparisons on eight sets of data. [Results] The results showed that the bats did not fly out the house for more than 30-minute with ‘20 watt bulb’ in the experiment of “Only light interference”, and they did not even fly during 2-hour period in ‘50 watt bulb’ experiment. The results of multiple comparative analysis showed that it took the shortest time for bats to fly out in the pattern of ‘no interference’ (5.14 ± 2.41 min on average), while the longest time was observed in the pattern of “repel + light”, (22.71 ± 2.06 min). Besides, there were no significant differences in the time among other five patterns (P > 0.05; 14.57 ± 7.91 min, 10.14 ± 2.67 min, 14.71 ± 8.71 min, 13.86 ± 7.69 min and 9.71 ± 2.69 min, respectively). [Conclusion] In conclusion, “having no interference” is the most secure and effective solution to drive the bats fly out the human houses. Our study provides a scientific and theoretical basis for the development of the coexistence between human and bats.