| 潘利文,王航俊,张钊,张永普,邹清.2022.浙江省发现文昌鱼.动物学杂志,57(5):742-750. |
| 浙江省发现文昌鱼 |
| Discovery of the Amphioxus in Zhejiang Province, China |
| 投稿时间：2022-02-16 修订日期：2022-08-29 |
| DOI：10.13859/j.cjz.202205010 |
| 中文关键词: 浙江省 文昌鱼 COI基因 |
| 英文关键词:Zhejiang Province Amphioxus COI gene |
| 基金项目:中央海岛和海域保护资金项目（财建〔2019〕191号） |
|
| 摘要点击次数: 112 |
| 全文下载次数: 852 |
| 中文摘要: |
| 2021年8月，在浙江省南麂列岛国家级海洋自然保护区发现国家Ⅱ级重点保护野生动物文昌鱼。对采集到的2尾成体文昌鱼（编号：ZJ 01♂，ZJ 02♀）进行了形态测量和线粒体COI基因序列分子系统关系重建，结合形态特征和分子测定结果，确定为文昌鱼科（Branchiostomatidae）文昌鱼属（Branchiostoma）的日本文昌鱼（B. japonicum）。经查阅相关文献资料，其为浙江省首次记录种，这将对文昌鱼资源的保护提供科学资料。 |
| 英文摘要: |
| [Objectives] Amphioxus is a transitional animal from lower invertebrates to higher vertebrates, which plays an extremely important role in the research of origin and evolution of vertebrates. Currently, the family Branchiostomatidae contains three genera: Branchiostoma, Asymmetron and Epigonichthys. In August 2021, when conducted a comprehensive survey in Nanji Islands National Marine Nature Reserve, Zhejiang Province, the author discovered amphioxus which is a national second-class protected animal. [Methods] Compared the specimens (one male ZJ 01♂, one female ZJ 02♀) with other species of amphioxus by comparing their morphological characteristics and measurement data (Fig. 1, Table 2). Phylogenetic analysis was performed on the basis of COI sequences of the samples and other amphioxus to determine the phylogenetic location of the specimens. The Kimura2-Parameter model in MEGA11.0.1 was used to calculate the genetic distances between species of the amphioxus. [Results] We found the specimens were similar with Branchiostoma japonicum in morphological. Based on molecular evidence, the specimens were clustered with the B. japonicum (Fig. 2). Genetic distance estimation based on Kimura2-Parameter model showed that the distance between the specimens and B. japonicum was the closest, ranging from 0.006 to 0.013, the distance much lower than that between other species range from 0.171 to 0.269 (Table 3). [Conclusion] Combined with morphological and molecular biological methods, this species was identified as B. japonicum. According to relevant literatures, the author presumed that amphioxus in Nanji Island were the pelagic larvae of amphioxus which came from Taiwan Warm Current in the coastal waters of Fujian province. It is the first recorded species in Zhejiang Province, which will provide scientific data for the protection of amphioxus resources. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
