| 周圣博,于梦竹,苗青,翟强,徐树军,关萍.2022.辽宁沈阳发现韩国林蛙.动物学杂志,57(6):944-950. |
| 辽宁沈阳发现韩国林蛙 |
| Rana coreana found in Shenyang, Liaoning |
| 投稿时间：2022-03-09 修订日期：2022-11-25 |
| DOI：10.13859/j.cjz.202206015 |
| 中文关键词: 韩国林蛙 辽宁省 蛙属 两栖动物 分布新记录种 |
| 英文关键词:Rana coreana Liaoning Province Rana Amphibians New record |
| 基金项目:2021年辽宁省普通本科高等学校校际合作项目-资源共享（2021-57），“辽宁盘锦湿地生态系统野外科学观测研究站”建设项目 |
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| 中文摘要: |
| 2021年11月在沈阳市辽中区蒲河湿地公园（41°30′55″ N，122°78′30″ E，海拔30 m）采集到2号无尾两栖类标本，经形态特征比较确认为无尾目（Anura）蛙科（Ranidae）蛙属（Rana）物种，基于线粒体16S rRNA基因对蛙属19个物种的亲缘关系及系统发育进行分析，其与韩国及中国山东文登昆嵛山分布的韩国林蛙（R. coreana）遗传距离最近，并在最大似然树中聚为一支，应属种内关系。综合形态分析和系统发育比较，确定采集到的标本为韩国林蛙，系辽宁省两栖动物分布新记录种。本次发现将辽宁省分布的蛙属物种增至5种。 |
| 英文摘要: |
| In November, 2021, 2 amphibians were collected from Puhe Wetland Park, Liaozhong District, Shenyang (41°30′55″ N, 122°78′30″ E, elevation 30 m). After comparison of morphological characteristics, they were confirmed as species of Rana, Ranidae and Anura.In order to determine the taxonomic relationship of this species and further determine their distribution characteristics, we conducted in-depth research and analysis on them. We used second high-throughput sequencing to sequence the mitochondrial 16S rRNA gene, and constructed a phylogenetic tree to analyze the phylogeny of 19 species of Rana (Table 1). This species of Rana has the closest genetic distance to R. coreana from Korea and Kunyu Mountain in Shandong Province, China, and clustered into a branch in maximum likelihood tree, which suggested an intraspecies relationship (Fig. 2 and Table 2). Based on morphological analysis and phylogenetic comparison, it was determined that the collected specimen was R. coreana (Fig. 1). R. coreana is a new record of amphibians in Liaoning Province. The discovery brings the number of frog species in Liaoning province to 5. |
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