Systematic survey on the species diversity and distribution of Chiroptera has been conducted in China recently. Therefore, extensive investigation should be performed to identify the diversity and distribution information of Chiroptera. In September 2021, we sampled eight Myotis bat individuals from Siyu Cave in Shidu, fangshan district, Beijing. Eight Myotis bats were identified as Chinese water myotis (Myotis laniger) based on morphological data, barcode sequence (COI and Cyt b) and phylogenetic reconstruction. This is the first record of Chinese water myotis in Beijing.