| 刘京,程彦林,周铝,姜世兴,周军健,李仕泽.2023.贵州威宁发现景东臭蛙.动物学杂志,58(1):148-156. |
| 贵州威宁发现景东臭蛙 |
| Odorrana jingdongensis Found in Weining, Guizhou Province |
| 投稿时间：2022-08-02 修订日期：2023-01-26 |
| DOI：10.13859/j.cjz.202301015 |
| 中文关键词: 景东臭蛙 分布新记录种 贵州省 |
| 英文关键词:Odorrana jingdongensis New record Guizhou Province |
| 基金项目:贵州省基础研究项目（黔科合基础-ZK〔2022〕一般540，黔科合基础〔2020〕1Y083），贵州省林业科技项目（黔林科合〔2020〕13号），贵州省教育厅青年科技人才成长项目（KY字〔2020〕234号），遵义市科技局项目（遵市科合HZ字〔2020〕319号） |
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| 中文摘要: |
| 2020年8月6日在贵州省威宁县雪山镇（27°04′09″ N，104°04′44″ E，海拔2 218 m）采集到6号两栖动物标本，经形态特征比较，与景东臭蛙（Odorrana jingdongensis）相似；基于线粒体16S rRNA构建臭蛙属（Odorrana）部分物种系统发育树，此次采集的臭蛙标本与景东臭蛙聚为一支，且具有较高的支持率（0.97/92，贝叶斯法/最大似然法）；基于Kimura双参数模型估算本次采集的臭蛙标本与景东模式产地标本间的遗传距离为0%，远小于臭蛙属物种间的遗传距离（1.3% ~ 9.5%）。综合形态特征和分子系统发育比较，确定此次采集到的臭蛙标本为无尾目（Anura）蛙科（Ranidae）臭蛙属的景东臭蛙，系贵州省两栖动物分布新记录种。 |
| 英文摘要: |
| During the amphibian survey in Xueshan Town, Weining County, Guizhou Province, China on 6 August, 2020, six specimens were collected (27°04′09″ N, 104°04′44″ E, altitude 2 218 m). Compared the specimens with other species of the genus Odorrana (Table 1) by morphological and molecular evidences, the specimens were identified as O. jingdongensis, which is the new record of Guizhou Province, China. Morphologically, we mainly compared measurement data (Table 2) and diagnosis characters (Fig. 1), the result showed that the specimens from Guizhou were similar to O. jingdongensis. Molecularly, we constructed Bayesian inference (BI) and maximum likelihood (ML) inference phylogenetic trees based on 16S rRNA gene and calculated the genetic distances between species of the genus Odorrana using Kimura two-parameter model in MEGA v6.0. The ML and BI trees of the mitochondrial DNA dataset presented almost consistent topology, both showed that the four specimens from Guizhou were clustered with the O. jingdongensis with a high support (0.97/92, BI/ML) (Fig. 2). The genetic distance between the specimens from Guizhou and O. jingdongensis from type locality was 0%, which is much lower than that between Odorrana species range from 1.3% to 9.5% (Table 3). |
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