| 李妹妹,罗平钊,陈德辉,史海涛,林柳.2023.基于DNA分子标记的潘氏闭壳龟遗传多样性分析.动物学杂志,58(1):126-134. |
| 基于DNA分子标记的潘氏闭壳龟遗传多样性分析 |
| Genetic Diversity Analysis of Pan’s Box Turtle Cuora pani Based on DNA Molecular Markers |
| 投稿时间：2022-08-30 修订日期：2023-01-24 |
| DOI：10.13859/j.cjz.202301012 |
| 中文关键词: 潘氏闭壳龟 线粒体Cyt b基因 ND4基因 R35基因 遗传多样性 |
| 英文关键词:Cuora pani mtDNA Cyt b gene ND4 gene R35 gene Genetic diversity |
| 基金项目:国家自然科学基金项目（No. 31960101，32170532） |
|
| 摘要点击次数: 108 |
| 全文下载次数: 694 |
| 中文摘要: |
| 潘氏闭壳龟（Cuora pani）是中国特有种，国家二级重点保护野生动物。由于非法贸易加之栖息地破坏等，其野外种群数量十分稀少。本研究旨在利用线粒体基因与核基因标记揭示潘氏闭壳龟的遗传多样性和遗传结构，以期为中国潘氏闭壳龟的种群保护和管理提供科学依据。本次共研究14只潘氏闭壳龟个体，其中，6只原产地为四川广元，8只产地未知。结果表明，在14只潘氏闭壳龟样本中鉴定出Cyt b单倍型、ND4单倍型和R35单倍型各2个，且均有1个新单倍型；Cyt b、ND4和R35基因单倍型多样性分别为0.440、0.143和0.154，核苷酸多样性分别为0.000 41、0.000 19和0.000 63；各单倍型的平均遗传距离（p）均小于0.01。基于三个基因联合数据集的系统发育分析表明，潘氏闭壳龟与金头闭壳龟（C. aurocapitata）互为单系进化支，且中性检测和核苷酸错配分析显示，潘氏闭壳龟群体近期历史上可能未经历群体扩张事件。潘氏闭壳龟群体遗传多样性低，种内变异小，群体应对环境变化的能力低，建议应加强对中国潘氏闭壳龟野外种群的基础研究和保护力度，同时规范人工繁殖，以避免近亲繁殖和种群衰退。 |
| 英文摘要: |
| [Objectives] The Pan’s Box Turtle (Cuora pani) is endemic to China and listed under level-II protection on the “List of Wildlife under Special State Protection”. Due to illegal trade and habitat destruction, its wild population is quite small. The aim of this study was to reveal the genetic diversity and genetic structure of C. pani by using mitochondrial and nuclear gene markers, so as to provide suggestions on population protection and management for C. pani in China. [Methods] In this study, Cyt b, ND4 and R35 genes were sequenced from 14 individuals, including 6 from Guangyuan, Sichuan and 8 of unknown origin. Nucleotide and haplotype diversity and group history dynamics of the samples were determined using DnaSP 5.0 software. The MEGA-X software was used to calculate the variable site, parsimony information site, genetic distance and base content of the sequence, and all haplotypes based on the Kimura 2-parameter model to build maximum likelihood tree. [Results] Results showed that two Cyt b, ND4 and R35 haplotypes were identified in the 14 samples and one new haplotype was identified in each gene (Table 3). The haplotype and nucleotide diversity of each gene marker were Cyt b (haplotype diversity 0.440, nucleotide diversity 0.000 41), ND4 (0.143, 0.000 19) and R35 (0.154, 0.000 63) (Table 4). The average genetic distance (p) of each haplotype was less than 0.01. Phylogenetic analysis based on the three combined gene datasets showed that C. pani and C. aurocapitata were monophyletic clades. Neutral detection and nucleotide mismatch analysis showed that C. pani populations may not have experienced population expansion events in recent history (Fig. 2). [Conclusion] The population of C. pani has low genetic diversity, small intraspecific variation and low ability to cope with environmental changes. Therefore, it is suggested that the basic research and protection of the wild population of C. pani in China should be strengthened, and artificial breeding should be regulated to avoid inbreeding and population recession. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
