上海市水产研究所 上海市水产技术推广站,上海市水产研究所 上海市水产技术推广站,上海市水产研究所 上海市水产技术推广站,上海市水产研究所 上海市水产技术推广站
上海市农委青年成长计划项目[沪农青字（2014）第3-11号]
Shang hai fisheries research institute,Shang hai fisheries technical extension ststion,Shang hai,200433,Shang hai fisheries research institute,Shang hai fisheries technical extension ststion,Shang hai,200433,Shang hai fisheries research institute,Shang hai fisheries technical extension ststion,Shang hai,200433,Shang hai fisheries research institute,Shang hai fisheries technical extension ststion,Shang hai,200433
- 摘要 | |
- 访问统计 |
- 参考文献 [14] |
- 相似文献 [20] | | |
- 文章评论
为确定导致鳜（Siniperca chuatsi）细菌性感染死亡的病原，从患病濒死的鳜肝中分离出一株优势菌B01，经人工回感实验确定其分离菌的致病性，并利用VITEK-2全自动微生物鉴定仪及16S rRNA序列分析对纯培养细菌进行鉴定。此外，对分离的菌株进行药敏实验。研究结果表明，菌株B01对鳜鱼具有致病性。经VITEK-2全自动微生物鉴定仪鉴定菌株B01为荧光假单胞菌（Pseudomonas fluorescens）（表1），并在紫外灯可以产生荧光（图1）。进一步的16S rRNA基因序列和系统发育树分析表明，该菌与荧光假单胞菌同源性达到了97%以上，并和（图2）。药物敏感性实验结果显示，该分离株对恩诺沙星、诺氟沙星、链霉素和四环素药物等6种药物高度敏感（表2）。
To determine the pathogenic bacterium infecting Siniperca chuatsi. Bacterium was isolated from the liver of diseased S. chuatsi and identified by the Vitek 2 Compact Identification System and 16S rRNA sequence analysis. Healthy S. chuatsi was used for experimental infection with bacterial suspension. A bacterial strain B01 was isolated from diseased S. chuatsi. Identification demonstrated that the bacterium was Pseudomonas fluorescens（Table1，Picture 2）, and it’s growth characteristics was aslo studied. And the results indicated the B01 could fluoresce under ultraviolet(Picture 1). Infection with the bacterial suspension to healthy S. chuatsi could reproduce the diseased symptoms as occurred naturally and the same bacterium could be recovered from these infected Siniperca chuatsi. The susceptibility test to antibiotics demonstrated that the bacterial strain B01 was susceptible to enrofloxacin, norfloxacin, streptomycin and tetracycline（Table2）. P. fluorescens is a pathogen for S. chuatsi.
引用本文
安伟,肖雨,高晓华,张明辉,张崇文,邵玲,何正侃.2014.鳜源致病性荧光假单菌的分离与鉴定.动物学杂志,49(5):760-765.
复制文章指标
- 点击次数:2744
- 下载次数: 2507
- HTML阅读次数: 0
历史
- 收稿日期:2014-03-11
- 最后修改日期:2014-08-27
- 录用日期:2014-08-21
- 在线发布日期: 2014-09-17
