Tawny puffer (Takifugu flavidus) is a species found in China considered to have potential for aquaculture. Through the analysis of the growth, survival, oxygen consumption, gill Na /K -ATPase, and liver antioxidase of young fish tawny puffer at different low salinities (0, 1.7, 5, 10, 15, and 20), the ability of tawny puffer young fish to adapt to the low salinity environment was studied. The effects of salinity on the indices were analyzed using the one-way ANOVA after arcsin-transformation of data in case of percentages, followed by Student-Newman-Keuls test (SNK). All analyses were performed with a significance level of p<0.05. The results showed that all tawny puffer young fish at salinity of 0 died 3 weeks after experiment, and young fish at salinity of 1.7 appeared a large number of death 6 weeks after experiment, the final survival rate was very low (17.33%), all fish lived at salinities of 5 to 20 in the whole experimental process. Although no significant difference was observed in total length specific growth rate among 1.7-20 salinities, body weight specific growth rate at 1.7 salinity was significantly lower than that at other salinity treatment group, there was no significant difference among 5-20 salinities, both the highest total length specific growth rate and the highest body weight specific growth rate were found at salinity of 10. Although no significant difference was observed in feed coefficient among 1.7-20 salinities at first six weeks, the highest and lowest feed coefficient were found at salinities of 1.7 and 10, respectively; In addition, no significant difference was observed in total feed coefficient among 1.7-20 salinities. Although no significant difference was observed in the oxygen consumption rate among 5-20 salinities, the lowest oxygen consumption rate was found at salinity of 10. The lowest gill Na /K -ATPase (NKA) activity was also found at salinity of 10; The relationship between salinity (5-20) and the NKA activity was modelled by a quadratic equation (y = 0.0832x2 - 2.1252x 20.915, r2 = 0.9779); From the equation, the lowest NKA activity was predicted to occur at salinity of 12.77. Although no significant difference was observed in liver superoxide dismutase (SOD) and glutathion peroxidase (GSH-PX) activities among 1.7-20 salinities, the liver catalase (CAT) activities at salinity of 10 and 15 were significantly lower than that at salinity of 1.7 and 20; The CAT activities showed a parabolic relationship with salinity (1.7-20)(y = 0.2577x2 - 5.8076x 87.357, r2 = 0.8771), the lowest CAT activity was predicted to occur at salinity of 11.27. Therefore, the critical survival low salinity of tawny puffer young fish is close to 1.7, the salinity of not less than 5 can be suitable for growth and survival of the young fish, the optimal salinity range for tawny puffer young fish is from 10 to 15. The results suggest that cultural salinity of tawny puffer young fish is not less than 5, and the best cultural salinity range is 10-15, Appropriate to reduce salinity in the cultural production of tawny puffer young fish is advantageous. The results of this study are useful in increasing the cultural production of the species