贵州大学动物科学学院水产科学系 贵阳 550025,贵州大学动物科学学院水产科学系 贵阳 550025,贵州大学动物科学学院水产科学系 贵阳 550025,贵州大学动物科学学院水产科学系 贵阳 550025,贵州大学动物科学学院水产科学系 贵阳 550025
国家自然科学基金项目（面上项目，重点项目，重大项目）
Department of Fisheries Science of Collage of Animal Sciences,Guizhou University,Guizhou Province Guiyang 550025,Guizhou university,Guizhou university,Guizhou university,Guizhou university
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本试验旨在探究普安银鲫(Carassius auratus gibelio)早期发育中氧化损伤程度和总抗氧化能力的变化,及维生素C(Vitamin C,Vc)对它们的影响。采用生化方法测定了普安银鲫早期发育中丙二醛(Malondialdehyde,MDA)含量、蛋白质羰基(Protein carbonyl,PCA)含量、乳酸脱氢酶(Lactate dehydrogenase,LDH)的活性和总抗氧化能力(Total antioxidative capacity,T-AOC)。结果显示:(1)普安银鲫早期发育中MDA、PCA的含量和LDH的活性均呈“升高-降低”的趋势,均在原肠中期达到最高,T-AOC逐渐增加；(2)30 mg/L的Vc溶液中仔鱼孵化率和成活率最高,分别高出对照组16.2%和17.8%；(3)30 mg/L的Vc组中MDA和PCA的含量及LDH的活性显著低于对照组(P<0.05),T-AOC显著高于对照组(P<0.05)。研究表明：(1)在普安银鲫胚胎期受到的氧化损伤比胚后严重；(2)适宜浓度的Vc添加到普安银鲫的孵化水体中能提高胚胎及仔鱼的抗氧化能力,有效降低胚胎及仔鱼受到的氧化损伤,提高其孵化率及存活率。
The bottleneck which is low hatching rate and low survival rate of larva restrict development of fish, in order to improve the rate of hatch and survival of larva, Taken Carassius auratus gibelio (C.auratus gibelio) as the main target, different concentration of vitamin C solution were added to hatching water of C. auratus gibelio, until the yolk sac disappeared completely. The concentration of vitamin c were 0 mg/L, 20 mg/L, 25 mg/L, 30 mg/L and 35 mg/L. The hatching rate and survival rate of larvae were recorded during the process of experiment, and the contents of malondialdehyde and protein carbonyl, the activity of lactic dehydrogenase and total antioxidant capacity during early development of C.auratus gibelio were analyzed, which immersed in optimal concentration of vitamin C group and control group. The results showed that: ⑴The rate of hatch and survival of larva increased when the concentration of vitamin C lower than 30 mg/ L, higher concentration of vitamin C solution(more than 30 mg/ L) restrained the rate of hatch and survival of larva, and the rate of hatch and survival of larva reached highest as the concentration of vitamin C was 30 mg/ L.⑵The contents of malondialdehyde, protein carbonyl and the activity of lactic dehydrogenase were showed “up-down”trend, and these three indexes reached hightest in mig-gastrocoel stage, while the total antioxidant capacity increased during early development of C.auratus gibelio.⑶The contents of malondialdehyde, protein carbonyl and the activity of lactic dehydrogenase of vitamin c group were significantly lower than that of control group, while the total antioxidant capacity significantly higher than that of control group(P<0.05). The research showed that: ⑴The oxidative damages during embryonic period were severely than that of postembryonic of C. auratus gibelio .⑵ Appropriate vitamin c solution ( 30 mg/L) vitamin C solution were added to hatching water could improve the antioxidant capacity during embryonic period, and effectively reduce the oxidative damage , and improve the rate of hatch and survival of larva.
引用本文
姚俊杰,熊铧龙,蒋左玉,安苗,朱忠胜.2015.维生素C对普安银鲫早期发育中氧化损伤及总抗氧化能力的影响.动物学杂志,50(4):581-590.
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- 收稿日期:2014-09-08
- 最后修改日期:2015-06-30
- 录用日期:2015-06-23
- 在线发布日期: 2015-07-16
