Abstract:The bottleneck which is low hatching rate and low survival rate of larva restrict development of fish, in order to improve the rate of hatch and survival of larva, Taken Carassius auratus gibelio (C.auratus gibelio) as the main target, different concentration of vitamin C solution were added to hatching water of C. auratus gibelio, until the yolk sac disappeared completely. The concentration of vitamin c were 0 mg/L, 20 mg/L, 25 mg/L, 30 mg/L and 35 mg/L. The hatching rate and survival rate of larvae were recorded during the process of experiment, and the contents of malondialdehyde and protein carbonyl, the activity of lactic dehydrogenase and total antioxidant capacity during early development of C.auratus gibelio were analyzed, which immersed in optimal concentration of vitamin C group and control group. The results showed that: ⑴The rate of hatch and survival of larva increased when the concentration of vitamin C lower than 30 mg/ L, higher concentration of vitamin C solution(more than 30 mg/ L) restrained the rate of hatch and survival of larva, and the rate of hatch and survival of larva reached highest as the concentration of vitamin C was 30 mg/ L.⑵The contents of malondialdehyde, protein carbonyl and the activity of lactic dehydrogenase were showed “up-down”trend, and these three indexes reached hightest in mig-gastrocoel stage, while the total antioxidant capacity increased during early development of C.auratus gibelio.⑶The contents of malondialdehyde, protein carbonyl and the activity of lactic dehydrogenase of vitamin c group were significantly lower than that of control group, while the total antioxidant capacity significantly higher than that of control group(P<0.05). The research showed that: ⑴The oxidative damages during embryonic period were severely than that of postembryonic of C. auratus gibelio .⑵ Appropriate vitamin c solution ( 30 mg/L) vitamin C solution were added to hatching water could improve the antioxidant capacity during embryonic period, and effectively reduce the oxidative damage , and improve the rate of hatch and survival of larva.
