The bullfrog （Rana catesbeiana）, which belongs to Anura, Ranidae and Rana, is one of the important economic farmed animals in China. The morphologic of the peripheral hematocytes in the bullfrog has not yet received enough attention. In this study, we observed the composition and morphology of the peripheral blood cells from 20 individuals (each 10 for male and female) using Wright's staining under the light microscope or the scanning electron microscopy (SEM). The diameters of various hematocytes and their nuclei in shortest and longest axises were measured using routine methods, and the experimental date were analyzed by SPSS19.0.The results showed that the peripheral hematocytes of the bullfrog were composed of erythrocyte, leukocyte, and thrombocyte. The mean size of erythrocytes in length × width was 25.68 ± 1.88 μm × 16.49 ± 1.53 μm (Table 1). The surface of erythrocyte was smooth under the SEM (PlateⅡ). Typically, Thrombocyte was ovoid or spindle in shape (PlateⅠ) and it was the smallest blood cell with mean size were 8.62 ± 1.04 μm × 7.47 ± 1.11 μm (Table 1). Leukocytes consisted of lymphocyte, monocyte, plasmacyte, neutrophil, eosinophil and basophil. Under the SEM, the surfaces of various leukocytes were rough with many irregular protrusions (PlateⅡ). Lymphocytes including small and large lymphocytes occupied higher percent, small lymphocytes were found approximately to be 32.66% ± 4.29% of the total leukocyte, large lymphocytes accounted for about 6% (Table 1). The number of the basophils was the least in the leukocytes, only accounted for about 4.78% ± 0.83% (Table 1). Plasmacytes were typically elliptical in shape (PlateⅠ), and their sizes varied obviously around the mean size 23.51 ± 0.59 μm × 22.86 ± 0.67 μm (Table 1). In addition, the quantitative proportion of the lymphocyte, basophil and plasmacyte and the size of lymphocyte and basophil were influenced by gender（P < 0.05）.