An important environmental selection pressure impending signal detection is ambient noise. Vertebrates use a number of strategies to avoid signal masking by anthropogrnic noise, including increasing call duration, frequency and amplitude. We tested wether traffic noise level influenced echolocation behavior in Vespertilio superans under nature condition. We recorded the echolocation calls emitted by two groups of bats which were flying in different traffic noise level. The results showed that only call frequency raised in respond to high traffic noise, including starting frequency (T test: p < 0.001), peak frequency (Mann-Whitney U: p < 0.001), ending frequency (Mann-Whitney U: p < 0.001) and bandwidth (T test: p < 0.05), instead of call duration (T test: p > 0.05) and slope (Mann-Whitney U: p > 0.05). These results indicate that the echolocation calls of Vespertilio superans have significant plasticity in traffic noise.