① 中国水产科学研究院淡水渔业研究中心 农业部淡水鱼业和种质资源利用重点实验室 无锡 214081,① 中国水产科学研究院淡水渔业研究中心 农业部淡水鱼业和种质资源利用重点实验室 无锡 214081,① 中国水产科学研究院淡水渔业研究中心 农业部淡水鱼业和种质资源利用重点实验室 无锡 214081,① 中国水产科学研究院淡水渔业研究中心 农业部淡水鱼业和种质资源利用重点实验室 无锡 214081;② 南京农业大学无锡渔业学院 无锡 214081
由中央级公益性科研院所基本科研业务费专项基金(2015JBFM16)和江苏省水产三项工程项目(201312)共同资助。
① Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,① Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,① Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,① Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences
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应用组织切片和透射电镜技术研究眼柄切除对加速克氏原螯虾（Procambarus clarkii）卵巢发育进程的影响。实验分成4组，选取眼柄切除15 d和30 d两个时间点进行。切除眼柄30 d后，雌虾体长增长显著，从（11.50 ± 2.30）cm增长至（18.20 ± 3.40）cm（P < 0.05），GSI指数从4.56% ± 2.81%增长至8.05% ± 2.51%（P < 0.05），体重和螯肢长也有所增长（P > 0.05）。电镜观察发现卵母细胞从发育Ⅱ期迅速度过Ⅲ期进入到Ⅵ期阶段，卵母细胞体积变大，变圆；液泡压缩变小；卵黄颗粒加速积累，逐渐占满整个胞内空间；滤泡细胞胞质从黏稠变稀薄。这些结果表明，切除眼柄在短期内显著加快卵母细胞内卵黄颗粒的积累，并且加快个体生长。
In this study, histologic methods were used to study the organizational structure of female ovary development induced by eyestalk ablation in red swamp crayfish (Procambarus clarkii). Total of 60 female crayfishes in phase II were selected and the experiment was divided into four groups as well as two time points (group 1: control for 15 days; group 2: control for 30 days; group 3: treatment for 15 days; group 4: treatment for 30 days). The data was analyzed using SPSS 19.0 statistical software. Our results show that 30 days after eyestalk ablation, the individual body-length increased significantly, from (11.50 ± 2.30) cm increased to (18.20 ± 3.40) cm(P<0.05)(Table 1); GSI index growth from 4.56% ± 2.81% to 8.05% ± 2.51% (P<0.05), as well as body-weight and chelicerae-long index also increased(P>0.05). 30 days after eyestalk ablation, the oocytes observed by electron microscope were quickly developed from phase II through stage III to VI stage(Table 2), Oocyte volume becomes larger, more rounded; Vacuole compressed smaller; yolk granules accumulated gradually accelerated, finally fill the entire intracellular space; cytoplasm in follicular cells becomes thinner(Figure 1). These results indicated that eyestalk ablation could significantly accelerate the yolk granules in oocytes of accumulation, and speed up the individual growth.
引用本文
水燕,汤雪君,徐增洪,周鑫.2016.切除眼柄对加速克氏原螯虾卵巢发育进程的组织学研究.动物学杂志,51(3):449-454.
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- 收稿日期:2015-04-28
- 最后修改日期:2016-02-22
- 录用日期:2015-12-28
- 在线发布日期: 2016-05-24
