In order to evaluate the characteristics of zooplankton community in Nansi Lake and the relationship between the zooplankton community and the water quality, samples were collected in July (summer) and December (winter), 2012, from Nansi Lake. Combined with historical data, the characteristics of zooplankton community in Nansi Lake and the temporal and spatial variation were described. Environmental factors of each sample site were also collected and regression analysis of the zooplankton community diversity and environmental factors was carried out. As a result, 163 zooplankton species were identified, including 78 Rotifera, 65 Protozoa, 17 Cladocera and 3 Copepoda. 141 zooplankton species were identified in summer, and 105 species were found in winter. Most of zooplankton species were found in the Weishan Lake, followed by Nanyang Lake, Zhaoyang Lake and Dushan Lake. The average density of zooplankton species in Nansi Lake is 2192 ind/L, and the average biomass is 2.27 mg/L. Most of the zooplankton species have higher density and biomass in summer than in winter except the Protozoa. The richness indexes, Shannon-Wiener indexes and evenness indexes in summer and winter of zooplankton community in Nansi Lake are 3.52 and 2.52,3.96 and 3.48,0.57 and 0.52, respectively. Stepwise regression analysis on the density, mass and diversity indexes with the environmental indexes indicated that, total nitrogen is the most important index affect on zooplankton density, total nitrogen and water temperature are most important indexes affect on zooplankton mass, and pH and conductivity are most important indexes affect on zooplankton diversity.