In order to obtain the salmon and trout seeds with the advantages of hybrid and triploid, steelhead trout (salmon gairdneri) and brook trout (Salvelinus fontinalis) reciprocal cross fertilized eggs of experimental induced by 6-dimethyl amino purine (6-DMAP) has been done. Hybridization results showed that the group eggs of brook trout (♀)×steelhead trout (♂) all died after 10 min after fertilization, and the development steelhead trout (♀)×brook charr (♂) fertilized eggs is normal, the triploid induction can be performed by 6-DMAP. With 6-DMAP in fertilized eggs result shows that the initial induction time for 12-15min, reached the highest eyed rate, 75.87%-76.64%, in the five initial induction time gradient groups, hatching rate between 86.44-90.31%, triploid rate between 92.45-95.55%, difference was not significant (P>0.05). The duration induced by 10-15min with 6-DMAP, reached the highest eyed rate 74.28-76.81%, in the five induced duration gradient groups, hatching rate between 87.28-90.24%, triploid rate between 93.67-96.25%, difference was not significant (P > 0.05). The fertilized eggs reach the highest eyed rate in induced concentration of 120-150 mg/L, 73.57-76.27%, in the five concentration gradient groups, the hatching rate between 88.57-90.03%, triploid rate between 92.56-96.38%, difference was not significant (P > 0.05).