The increase of tourism has taken an amount of people and vehicles in the recent decade to the Nature Reserves in which wildlife inhabit. The unreasonable behaviors of tourists and vehicle noise have produced an displeasure pressure on the survival and reproduction of some endangered species. Understanding the way of tourism impacts on wildlife is a basis for the nature reserve managers to make a reasonable conservation strategies for the wildlife species under the tourist pressure. Unfortunately, few field studies related to this problem has been conducted in the nature reserve. In this paper we evaluated the reaction of Giant Panda (Ailuropoda melanoleuca) to the tourists by comparing the mean and minimum distance from the signs of Giant Panda presence to hiking trails and vehicle roads located in the year 2000 before the tourism development with those found in the year 2012 when the tourists were crowd in the summer at the Tangjiahe National Nature Reserve. The Giant Panda showed significant avoidance from both trails and roads (P = 0.024, Table 1). The first least avoiding distance threshold was 0﹣57 m and 0﹣460 m for hiking trails and roads, the second threshold was 57﹣800 m and 460﹣1 000 m, and the third one was 800﹣1 400 m and ＞1 000 m respectively. By the Mann-Whitney U test, we found that the mean signs of Giant Pandas in every 100 m across the distance to the haiking trails and driving roads showed a significant difference (1.9 ± 0.23 vs. 0.8 ± 0.15, Z =﹣3.48, P = 0.000) between the year 2000 and 2012. Meanwhile, the distance from the location of Giant Panda presence to the hiking trails and roads in every 100 m altitude range varied significantly (t = 3.76, P = 0.003, Table 2). The minimum avoiding distance occurred at altitude 2 000﹣2 100 m for the driving roads, while this distance for the hiking trails were at the altitude 2 100﹣2 200 m. Our data revealed the minimum safe distance which produce minimum influence on the Giant Panda by the tourists. Our approach that evaluated the influence of tourism for the Giant Panda can be used in the other nature reserves when they consider to accept the tourists.