A hibernating male of barbastelle was found in Huangbeijiao segment of the diversion canal in Shaoyuan Town (35°15′8.4″N, 112°6′34.5″E, 658 m elevation), Jiyuan City, Henan Province, in February, 2016. This sample was identified as Asian Barbastelle (Barbastella leucomelas) based on its external morphology, skull characteristics and molecular data. The body of specimen is at medium size with 40.4 mm for forearm length. The bat has triangular tragi and nearly square outline of ears with transverse ridges (Fig. 1). The ears join across the forehead without the prominent projecting lobe, which is a feature specific to B. leucomelas (Fig. 1). The skull is flattened, and the greatest length skull is 14.9 mm which is a bit smaller than B. beijingensis (Table 1). The dental formula of specimens is 2.1.2.3/3.1.2.3 = 34 (Fig. 2). Maximum-likehood trees based on Cyt b and ND1 gene sequences were constructed, respectively. Both of the cladograms indicated that this bat specimen is as B. leucomelas, rather than B. beijingensis (Fig. 3). The discovery of B. leucomelas in Jiyuan City added a new species record of Chiroptera for Henan Province, China. The specimen was preserved in Institute of Resources and Environment, Henan Polytechnic University.