附藓蛭态轮虫分离法的比较与分离条件的探索
作者:
作者单位:

中山大学生命科学学院,中山大学生命科学学院

基金项目:

国家自然科学基金项目(No. 31030015)


Comparative Studies on the Extraction Methods and Conditions of Moss-dwelling Bdelloidea Rotifers
Author:
Affiliation:

School of Life Sciences,Sun Yat-sen University,School of Life Sciences,Sun Yat-sen University

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [31]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    蛭态轮虫是附藓无脊椎动物中常见的类群,由于个体较小,观察难度大,尤其是对其分离困难,故对此类群的研究一直落后于其他轮虫类群。本文以文献报道附藓蛭态轮虫的分离方法为基础,设定了不同的实验组,比较了不同分离方法的分离效率,并探讨了不同分离条件对附藓蛭态轮虫分离效率的影响。结果显示:与简单的震荡复苏法相比,蔗糖震荡复苏法可分离出更多的蛭态轮虫,且在分离条件为pH 7.8、温度20℃和湿度60%时可达到最高的分离效率。文中还就各分离条件对分离效率的影响进行了讨论。

    Abstract:

    Bdelloidea rotifers are the common invertebrates in moss fauna. However, Bdelloidea rotifers is difficulty to observe due to the smaller size of each in individual and also it is difficulty to separate them. Therefore the research on this group invertebrate was behind compared to other rotifer taxa. This article is aimed at comparing the extraction efficiency on Bdelloidea rotifers by different extraction methods and exploring the affect on extraction efficiency under different experiment condition. The multi-shock resuscitation methods and the sucrose shock resuscitation methods were selected according to the literature reports on extraction methods of moss-dwelling Bdelloidea rotifer. Impact factors, such as pH, temperature and humidity were chosen as different experimental conditions, and the experiment was done by setting up the different experimental groups with Orthogonal table of L9 (Table 1). All the data obtained from the experiment were deal with Excel 2010 and the statistic analysis was processed with SPSS 19.0 for analysis of variance to determine the difference among the experiment results. The study results showed that the average number of Bdelloidea rotifers were 94.68 ind/g and 165.33 ind/g by multi-shock and sucrose shock resuscitation methods, respectively (Fig.1). The sucrose shock resuscitation methods could extract more Bdelloidea rotifers compared with the multi-shock resuscitation methods significantly (F = 24.017, P< 0.05). The influence of the extraction conditions on the extraction efficiency was in the order: pH > humidity > temperature. Both range analysis and variance analysis displayed that while the maximum extraction efficiency can be expected under the following extraction conditions: pH at 7.8, temperature at 20℃ and humidity at 60% respectively (Table 2﹣4).

    参考文献
    尹文英. 1992. 中国亚热带土壤动物[M]. 北京: 科学出版社.
    陈灵芝. 1993. 中国的生物多样性: 现状及其保护对策[M]. 北京: 科学出版社.
    何强, 杜桂森. 2004. 苔藓植物多样性保护现状[J]. 首都师范大学学报(自然科学版), 9(3): 52-57.
    杨再超, 王智慧, 张朝晖. 2009. 金矿区苔藓结皮表土原生动物群落与环境因子的关系[J]. 生态学杂志, 28(8): 1525-1530.
    杨潼. 2008. 中国东部武夷山风景区苔藓中的缓步动物[J]. 动物分类学报, 33(2): 348-353.
    查广才. 1998. 苔藓土壤动物种群动态的初步研究[J]. 信阳师范学院学报(自然科学版), 1(1): 61-66.
    查广才, 梁来荣, 周昌清. 2003. 苔藓土壤节肢动物群落及其多样性. 生态学报, 23(6): 1057-1062.
    席贻龙, 黄祥飞. 2001. 不同培养条件下萼花臂尾轮虫休眠卵的萌发[J]. 动物学报, 47(3): 292-297.
    徐润林编著. 2013. 动物学. 北京: 高等教育出版社.
    潘林, 焦德志, 王文峰, 等. 2010. 扎龙湿地苔藓群落土壤动物的分布及多样性[J]. 土壤, 42(4): 536-540.
    Crowe J H, Madin K A. 1975. Anhydrobiosis in nematodes: evaporative water loss and survival[J]. Journal of Experimental Zoology, 193: 323-334.
    Crowe J H, Hoekstra F A, Crowe L M. 1992. Anhydrobiosis[J]. Annual Review of Physiology, 54: 579-599.
    David H A. 1951. Further application of range to the analysis of variance[J]. Biometrika, 38: 393-407.
    Davis R C. 1981. Structure and function of two Antarctic terrestrial moss communities[J]. Ecological Monographs, 51: 125-143.
    Devetter M. 2010. A method for efficient extraction of rotifers (Rotifera) from soils[J]. Pedobiologia, 53: 115-118.
    Donner J. 1965. Ordnung Bdelloidea[M]. Bestimmungsbucher zur Bodenfauna Europas 6. Berlin: Akademie Verlag.
    Glime J M. 2013. Invertebrates: Introduction. Chapt. 4-1[M]. In: Glime J M. Bryophyte Ecology. Volume 2. Bryological Interaction. Michigan Technological University and the International Association of Bryologists.
    Jacobs M H. 1909. The effects of desiccation on the rotifer Philodina roseola[J]. Journal of Experimental Zoology, 6: 207-263.
    Kosanke J W, Osburn R M, Shuppe G I, Smith R S. 1992. Slow rehydration improves the recovery of dried bacterial populations[J]. Canadian Journal Microbiology, 38: 520-525.
    Merrifield K, Ingham R E. 1998. Nematodes and other aquatic invertebrates in Eurhynchium oreganum (Sull.) Jaeg., from Mary's Peak, Oregon Coast Range[J]. Bryologist, 101: 505-511.
    Mieczan T. 2009. Ciliates in Sphagnum peatlands: vertical micro?distribution, and relationships of species assemblages with environmental parameters[J]. Zoological Studies, 48: 33-48.
    ?rstan A. 1998. Factors affecting long-term survival of dry bdelloid rotifers: a preliminary study[J]. Hydrobiologia, 387/388: 327-331.
    Peters U, Koste W, Westheide W. 1993. A quantitative method to extract moss-dwelling rotifers[J]. Hydrobiologia , 255/256: 339-341.
    Ricci C. 1983. Life histories of some species of Rotifera Bdelloidea[J]. Hydrobiologia, 104: 175-180.
    Ricci C. 1984. Culturing of some bdelloid rotifers[J]. Hydrobiologia, 112: 45-51.
    Ricci C. 1987. Ecology of bdelloids: how to be successful[J]. Hydrobiologia, 147: 117-127.
    Ricci C. 1992, Rotifers: parthenogenesis and heterogony[C]. In Dallai R (ed.), Sex Origin and Evolution. Selected Symposia and Monographs UZI, 6: 329-341.
    Ricci C, Pagani M, Bolzern A M. 1989. Temporal analysis of clonal structure in a moss bdelloid population[J]. Hydrobiologia, 186/187: 145-152.
    Thorp J H, Covich A P. 2010. Ecology and Classification of North American Freshwater Invertebrates[M]. Academic Press, Boston.
    Womersley C Z. 1987. A reevaluation of strategies employed by nematode anhydrobiotes in relation to their natural environment[C]. In Veech J, Dickson D W. (eds), Vistas on Nematology. Soc. Nematol, 165-173.
    Wright J C, West P, Ramlov H. 1992. Cryptobiosis in Tardigrada[J]. Biology Review, 67: 1-29.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

尹志伟,徐润林.2017.附藓蛭态轮虫分离法的比较与分离条件的探索.动物学杂志,52(4):708-716.

复制
文章指标
  • 点击次数:1695
  • 下载次数: 1931
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2016-06-27
  • 最后修改日期:2017-06-14
  • 录用日期:2017-05-28
  • 在线发布日期: 2017-07-17