The aim of the present study was to explore the application of digital subtraction angiography(DSA) intubation technique in the dynamic acquisition of cerebrospinal fluid in Ganges River Monkey(Macaca mulatta). A total of 28 monkeys were randomly divided into two groups: experimental group and control group, 14 individuals each. Each monkey was injected with 3% pentobarbital sodium solution through left anterior limb vein for general nesthesia, and then hairs on the back around the spine were eliminated after disinfection. In the experimental group, lumbar puncture was started with the DSA targeting, and the control group lumbar puncture was directly performed. The epidural anesthesia catheter was sent from thelumbar subarachnoid space to the occipital cistern, in which the pump was buried for all 28 monkeys. The data were analyzed with Chi-square test. Modified Tarlov reference criteria were used for neural function evaluation, and a modified incision healing grade standard was also used for healing evaluation, using Spss17.0 software for data analysis. In the control group, we obtained 8 cases of success and 6 cases of failure,with no cerebrospinal fluid outflow in all animals. In the experimental group, the lumbar puncture was successful. All the pumps were able to collect colorless and transparent cerebrospinal fluid. After surgery, all the Ganges River Monkeys were returned to normal on the same day. With DSA intubation technique, the operation is simple, safe, and feasible with high success of erebrospinal fluid collection. By embedding the pump, quick and repeated acquisition of cerebrospinal fluid can be realized when the animals are awake and in full physiological state, which will help to improve the welfare of nonhuman experimental primate animals.Sampling catheters are placed in the cisterna, which will guarantee the quality of collected cerebrospinal fluid,the convenience of studying pathogenesis of ischemic stroke, thus this approach has a certain practical value.