Abstract:Sixty six infrared cameras were placed in 22 water sources from April to November 2014 to monitor the activity rhythms and time budgets of reintroducing Equus przewalskii in the Mount Kalamaili Ungulate Natur Reserve (MKUNR). Of 16 cameras that were placed in 8 water sources used frequently by the horses(Fig. 1) obtained obtain 2 051 independent photos on the reintroduced horse during 1 068 days. By analyzing those photos we found out the number of effective photos capturing the horses in the water source area was following the normal distribution. The difference of daily activity α index (Fig. 2) and diurnal β index (Fig. 3) of the horses showed significant difference between months (α index, t = 8.364, df = 7, P < 0.01. β index, t = 19.519, df = 7, P < 0.01), and the β index was 0.66 > 0.54, which indicated that reintroducing horseswas dominated in activity by daytime. Significant difference was detected in the seasonal activity index (single sample K-S test, t = 7.851, df = 23, P < 0.01) for the horse. However, no significant difference was found in the seasonal activity intensity index (Fig. 4) (c2 = 0.162, df = 2, P> 0.05) by the independent multi sample Kruskal-Wallis test. The results of multiple comparisons showed that the number of effective photosynthetic rate on the reintroducing horse in spring was significantly lower than that in summer and autumn (P < 0.05), but there was no significant difference between summer and autumn (P > 0.05). The reintroducinghorses were found in the water source at any time of day with a significantly variation between the groups sizes in the day time and night time (P< 0.05, T test), even in different hours. The water demands increased as the temperature increase and peaked around noontime (Fig. 5). The numbers of valid photo every day at different hours met the normal distribution for each season, and the horses at the water source showed a stable daily activity rhythm with a typical bell-shaped time allocation. In summer, the horses drank water at (25.29 ± 2.9) times/d and they took water is far less (13.88 ± 1.8) times/day than they did in spring (P < 0.01) by multiple comparison test. No any significant difference in the frequency of drank water between summer and autumn (P > 0.05). The number of horse drinking peaked from 13:00 to 17:00, 20:00﹣5:00 when the rapid decline, and 3:00﹣4:00 to the lowest, in the 5:00﹣13:00 when the rapid rise (Fig. 6). This study was firstly got the water use data in detail for the reintroducing horses by the real-time monitoring at the fixed water source in the MKUNR.