Loach, Paramisgurnus dabryanus, a small-sized fish belonging to the family of Cobitidae of the order Cypriniformes, can breathe air through its mouth when swimming to the water surface. And its accessory breathing organ for O2 and CO2 exchange is its posterior intestine. To investigate the effect of respiratory metabolism and oxidation resistance of gill and intestine as well as related physiological feedback mechanism in P. dabryanus, an air-breathing model under intestine breathing restrain treatment was employed. Mature individuals were chosen for intestine respiratory restriction by limiting them under the water surface. The total static metabolism rate, respiratory rate, lactic dehydrogenase activity (LDH), succinate dehydrogenase activity (SDH), Na+/K+ATPase activity (NKA), catalase activity (CAT) and superoxide dismutase (SOD) activity of gill and intestine were examined after 2 weeks′ acclimation in control and treatment groups. Data were expressed as Mean ± SE. All the measured data were analyzed and compared with one-way ANOVA and t-test using spss 19.0 and excel. The level of significant difference was set at P < 0.05. The result showed that total static metabolism rates in control and treatment group did not differ significantly (P > 0.05) (Table 1). However, the gill of P. dabryanus in treatment group breathed more quickly than the control group (P < 0.05) (Table 1). In treatment group, succinate dehydrogenase activity of gill showed a significant increase (P < 0.05), while succinate dehydrogenase activity and Na+/K+ATPase activity of posterior intestine showed a significant decrease (P < 0.05) (Fig. 1b, c). At the same time, lactic dehydrogenase activity of posterior intestine increased significantly in treatment group (Fig. 1a). Although there was no significant difference in catalase or superoxide dismutase activity in the gill, anterior and middle intestine between the control and treatment group (P < 0.05), the enzyme activity of posterior intestine in treatment group was lower than that of control group (P < 0.05) (Fig. 2a, b). When P. dabryanus was intestine breathing restricted, it would enhance its gill aerobic respiratory metabolism as an offset for intestine breathing loss, in order to meet physiological requirements and its air-breathing posterior intestine would have an oxidative stress response. Respiratory metabolism of the gill and posterior intestine of air-breathing loach P. dabryanus could be adjusted to adapt environment under different environment stress conditions.