For exploring the differences of serum biochemical indices for seven captive cranes species (Gruiformes), including Siberian Crane (Grus leucogeranus), Red-crowned Crane (G. japonensis), Black-crowned Crane (Balearicapavonina), Black-necked Crane (G. nigricollis), Grey-crowned Crane (B. regulorum), Blue Crane (Anthropoidesparadiseus) and White-naped Crane (G. vipio), ten biochemical indicators including total cholesterol, triglyceride and serum glucose were measured in this study (Table 1). Then, these measured values were compared with the average values provided by International Species Information System (ISIS) (Table 2). All data were determined for assumption of normality by Shapiro-Wilk test and homogeneity of variance by Welch′s test. One sample T-test was used to compare with the average value of ISIS. LSD or Tamhane′s test was performed in different crane species, depending on the variances. The results indicated that, 1) there was significant difference on total cholesterol, however, no significant difference on triglyceride and serum glucose among these seven crane species. Except for total cholesterol of Black-crowned Crane, total cholesterol of Grey-crowned Crane and serum glucose of Blue Crane, other indicators are higher than the average value of ISIS (Table 2). 2) There are significant differences in the concentration of total serum protein, albumin and globulins among these seven cranes species, with all these biochemical indicators in the range of ISIS (Table 3). 3) There are no significant differences in the level of serum creatinine and uric acid, with the concentration of uric acid lower than the average value of ISIS, however, there is significant difference of the urea nitrogen among these seven crane species (Table 4). The significant differences of serum biochemical indicators among crane species were mainly caused by feed and environmental factors. So the biochemical indicators of cranes provide an important reference for nutritional situation and healthy judgment, also a guiding role to breeding management for the captive animals.