In order to explore the effects of packing transport stress on antioxidant system in liver and muscle and effects of anti-stress agent in juvenile Eleutheronema tetradactylum, various groups including control group (without transportation), blank group (transported but without anti-stress agent), vitamin C group (vitamin C was added as an anti-stress agent) and glutamine group (glutamine was added as an anti-stress agent) were set in this study. Liver and muscle samples of juvenile E. tetradactylum were collected at 2 h, 6 h, and 9 h of transport stress to determine the related indexes. Tissue samples were preserved in liquid nitrogen immediately. The samples were added with normal saline according at a ratio of 1︰9, then they were made into 10% tissue homogenate after crushing and centrifugation. Measured items included superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), glutathione (GSH), total antioxidant capacity (T-AOC) in liver and muscle. Results showed that transport stress led to rapid increase in SOD activity in liver and muscle in blank group, however, SOD activity was significantly (P < 0.05) lower than that of the control group in vitamin C group and glutamine group, while there was no significant difference (P > 0.05) between the latter two groups (Fig. 1). Transport stress led to significant increase in the activity of CAT (P < 0.05) in liver and muscle in blank group, although slightly decreased later, the activity values were significantly higher than those in control group (P < 0.05) finally; however, these values in vitamin C group and glutamine group were not significantly different compared with the control group (P > 0.05) or slightly higher than that in control group (Fig. 2). Transport stress led to significant increase in the content of MDA (P < 0.05) in muscle and liver in blank group, but this result was alleviated significantly in vitamin C group and glutamine groups (Fig. 3). The contents of GSH showed different change trends between liver and muscle in different groups, and this might be related to their different physiological functions (Fig. 4). The values of T-AOC in liver and muscle were significantly higher in blank group than that in control group (P < 0.05), while the values were slightly higher in vitamin C group and glutamine groups than that in control group (Fig. 5). The analysis of antioxidant system of juvenile E. tetradactylum under the transport stress and the effects of anti-stress agent will provide reference for promoting artificial breeding of E. tetradactylum.