Abstract:Study on the hematology can not only help to understand the physiological and pathological variations of animals, but also detect the adaptation of animals to the environment. However, studies on the hematology of amphibians, especially in the Urodela, are limited. Here, the blood cell composition and hemoglobin concentration of Batrachuperus londongensis were researched in 27 individuals. Comparison between sexes was conducted by Independent-Samples T Test or Mann-Whitney U, and correlations between snout-vent length and body mass (Table 1) and hematological parameters were determined by linear regression. All statistical tests were performed with SPSS software. The mean value of hemoglobin concentration was 4.16 × 10-2 g/ml; erythrocytes were oval with ellipsoidal nucleus that was located in the center or eccentric position of the cell (Fig. 1), and mean erythrocyte count was 6.04 × 104 cell/mm3; the number of leucocytes was small, and its mean count was 2.90 × 103 cell/mm3 (Table 2). The lymphocytes were richest leucocytes, followed by monocytes, basophils, neutrophils, and eosinophils (Table 2). Among the hematological parameters, only the percentage of neutrophils and basophils showed significant sexual difference. Correlations between snout-vent length, body mass and hematological parameters were not significant (Table 3). Meanwhile, comparison between our results with those of other hynobiid salamanders was carried out. These basic data of blood cell component and hemoglobin concentration reported in this study are useful in the health monitoring and diagnosis, and in understanding the evolution and adaptation of B. londongensis.