Northern Three Toed Jerboa (Dipus sagitta) is one of dominant rodent pests in the western desert in Inner Mongolia. Study on home range of D. sagitta have theoretical and practical significance in exploring rodent reproductive strategies and the population dynamics. An experiment was conducted in Alax desert in order to investigate home range of D. sagitta subjected to successive grazing during April to October between the year of 2012 to 2014. Rodent was captured by living trapping method. The influence of grazing treatment on the home range of Northern Three Toed Jerboa between each gender and seasons, were tested using mixed effect model. Relation between plant factors and body mass within the home range was analyzed by partial redundancy analysis method. The results showed that the population density of D. sagitta in grazing exclusion sites was higher than that in successive grazing sites (Fig. 1). Successive grazing decreased height, coverage, density and biomass of herbage and shrub height significantly (Fig. 2). The home range of females in the field with successive grazing was larger significantly than that in grazing exclusion sites only on the month June (P < 0.05, Fig. 3a). The home range of males in grazing exclusion sites was significant larger than that in successive grazing sites on July (P < 0.05, Fig. 3b). No any remarkable difference was found between the habitat treatments in other seasons (P > 0.05, Fig. 3b). Although the treatment of successive grazing had no significant effect on the male′s home range (P > 0.05), it did extended the female′s home ranges (P < 0.05, Fig. 4, Table 2). Meanwhile, home ranges, both male and female were positive associated with the biomass of annual herbage (Fig. 5). It indicated that the home range of female jerboas was more sensitive to the food availability decreasing than males, especially during lactation period. While breeding was one important factors influencing the home range size for males. The Resources defending Hypothesis could be one for the home range change of D. sagitta due to grazing reducing the food availabilty.