The distribution and types of goblet cells in the intestine of Tilapia Galilaea (Oreochromis niloticus ♀ × Oreochromis aureus ♂) were studied by Alcian Blue-Periodic Acid Sthiff (AB-PAS) and Aldehyde Fuchsin-Alcian Blue (AF-AB) staining. The results showed that the intestinal mucosal layer of tilapia was thick, and a large number of intestinal villi decreased gradually from foregut to hindgut. Goblet cells were mainly located between the epithelial cells of intestinal villi, and their shape was mostly oval or approximately circular, and some were in a shape of a long and thin spindle (Fig. 1). The statistical analysis showed that the number of goblet cells per unit area from foregut to hindgut gradually increased, the average numbers of goblet cells in foregut, midgut and hindgut of were 230.0 ± 4.9 cell/mm2, 287.9 ± 7.5 cell/mm2 and 336.7 ± 10.0 cell/mm2, respectively, with significant difference among the three groups (P < 0.05, Table 1). PAS and AB-PAS staining showed that the goblet cells in tilapia intestinal were mainly neutral and acid double staining, the acid goblet cells were low in the intestinal segments (Fig. 2, 3), and the sulfuric acid cells were mainly sulfuric acid cells in AF-AB staining (Fig. 3), and the cups of goblet cells were lack of carboxylic acid.